A solution to the biophysical fractionation of extracellular vesicles: Acoustic Nanoscale Separation via Wave-pillar Excitation Resonance (ANSWER)
In: Sci Adv, 2022
academicJournal
Zugriff:
High-precision isolation of small extracellular vesicles (sEVs) from biofluids is essential toward developing next-generation liquid biopsies and regenerative therapies. However, current methods of sEV separation require specialized equipment and time-consuming protocols and have difficulties producing highly pure subpopulations of sEVs. Here, we present Acoustic Nanoscale Separation via Wave-pillar Excitation Resonance (ANSWER), which allows single-step, rapid (<10 min), high-purity (>96% small exosomes, >80% exomeres) fractionation of sEV subpopulations from biofluids without the need for any sample preprocessing. Particles are iteratively deflected in a size-selective manner via an excitation resonance. This previously unidentified phenomenon generates patterns of virtual, tunable, pillar-like acoustic field in a fluid using surface acoustic waves. Highly precise sEV fractionation without the need for sample preprocessing or complex nanofabrication methods has been demonstrated using ANSWER, showing potential as a powerful tool that will enable more in-depth studies into the complexity, heterogeneity, and functionality of sEV subpopulations.
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A solution to the biophysical fractionation of extracellular vesicles: Acoustic Nanoscale Separation via Wave-pillar Excitation Resonance (ANSWER)
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Autor/in / Beteiligte Person: | Zhang, Jinxin ; Chen, Chuyi ; Becker, Ryan ; Rufo, Joseph ; Yang, Shujie ; Mai, John ; Zhang, Peiran ; Gu, Yuyang ; Wang, Zeyu ; Ma, Zhehan ; Xia, Jianping ; Hao, Nanjing ; Tian, Zhenhua ; Wong, David T. W. ; Sadovsky, Yoel ; Lee, Luke P. ; Huang, Tony Jun |
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Zeitschrift: | Sci Adv, 2022 |
Veröffentlichung: | American Association for the Advancement of Science, 2022 |
Medientyp: | academicJournal |
DOI: | 10.1126/sciadv.ade0640 |
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