6xHis-CBU0823 failed to produce detectable lactate in in vitro assays.
2021
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Zugriff:
(A) Addition of 6xHis-oMLE produced significantly more pyruvate than 6xHis-CBU0823, and both produced significantly more than negative control and blank reactions. (B) Only 6xHis-oMLE yielded lactate amounts significantly above blank. Malic enzyme activity assays (3 mM malate, 5 mM NAD + and 1 mM MnCl 2 ) were started with either 54 μg 6xHis-CBU0823, 26 μg 6xHis-oMLE, or equivalent volume of mock purification (negative control) or PBS (blank). Reactions were stopped after 20 minutes. Each reaction was analyzed by GC-MS and mean values compared (* p < 0.05, ** p < 0.01, *** p < 0.001). Error bars represent standard deviation from the mean from six independent replicates.
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6xHis-CBU0823 failed to produce detectable lactate in in vitro assays.
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Autor/in / Beteiligte Person: | Janine Hofmann (11273418) ; Mebratu A. Bitew (11273421) ; Miku Kuba (11273424) ; David P. De Souza (2793088) ; Hayley J. Newton (8692020) ; Fiona M. Sansom (8893082) |
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Veröffentlichung: | 2021 |
Medientyp: | Bild |
DOI: | 10.1371/journal.pone.0255925.g007 |
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