Figure 6—figure supplement 1. Cross-linking times depend on the length of the bis-MTS cross-linker, functional state of the receptor and the presence Stargazin. ; (A) Trapping speed of M6M for active V666C receptors (empty circles) and desensitized in the presence (black diamonds) and absence (black circles) of Stargazin (Stg). Receptors were exposed to 1 μM M6M for 1 min intervals (as described Figure 2F and Materials and methods), with up to six sequential applications. Current reduction was determined after each 1-min exposure, averaged across patches and plotted here vs. cumulative trapping time. Current decay was monoexponential in every case, with τ indicated in brackets. (B) Same as in (A), but for M10M. (C) Trapping speed of M1M for active V666C receptors in the presence (black diamonds) and absence (empty circles) of Stg. (D) Same as in (C) but for M8M.

Figure 6—figure supplement 1. Cross-linking times depend on the length of the bis-MTS cross-linker, functional state of the receptor and the presence Stargazin. ; (A) Trapping speed of M6M for active V666C receptors (empty circles) and desensitized in the presence (black diamonds) and absence (black circles) of Stargazin (Stg). Receptors were exposed to 1 μM M6M for 1 min intervals (as described Figure 2F and Materials and methods), with up to six sequential applications. Current reduction was determined after each 1-min exposure, averaged across patches and plotted here vs. cumulative trapping time. Current decay was monoexponential in every case, with τ indicated in brackets. (B) Same as in (A), but for M10M. (C) Trapping speed of M1M for active V666C receptors in the presence (black diamonds) and absence (empty circles) of Stg. (D) Same as in (C) but for M8M.