HIV-1 viral spread in CEM IPMK or IPPK KO clones.
2021
Bild
Zugriff:
A and B. Top panel: Diagram of IPPK or IPMK , respectively. Red arrows denote approximate Cas9 cleavage site. Genomic PCR primer locations are indicated in black and orange. Primer locations are not exact. IPMK exon 1 expected PCR product size: 278 bp. IPPK exon 1 expected PCR product size: 355 bp. IPPK exon 1 to 7 fusion allele expected PCR product size: 397 bp. IPMK exon 1 to 6 fusion allele expected PCR product size: 252 bp. Bottom panels: Amplified genomic DNA from the designated cell lines run on an ethidium bromide stained agarose gel. C and D. Toluidine blue stained polyacrylamide gel of TiO 2 enriched inositol phosphates. E and F. HIV-1 p24 release graphed versus time for the specified HIV-1 NL4-3 -infected CEM cell lines. Each point represents the average of 4 independent infections. Asterisks signify significance from WT CEM cells. G and H. CD4 expression by flow cytometry in the indicated WT and KO CEM clones graphed on a box and whiskers. Box shows the 25 th to 75 th quartiles with the line denoting the median. Dots denote outliers. Significance levels: * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001. (TIF)
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HIV-1 viral spread in CEM IPMK or IPPK KO clones.
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Autor/in / Beteiligte Person: | Gregory A. Sowd (10017855) ; Christopher Aiken (47132) |
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Veröffentlichung: | 2021 |
Medientyp: | Bild |
DOI: | 10.1371/journal.ppat.1009190.s004 |
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