CUN induces cell ferroptosis by upregulation of lipid peroxidation and iron accumulation.
2022
Bild
Zugriff:
A and B: The levels of lipid ROS were evaluated in MDA-MB-453 and MCF-7 cells treated with CUN for 48 h or erastin for 2 h by flow cytometry using C11-BODIPY; C: The MDA accumulation was examined by a lipid peroxidation assay kit; D: The intracellular Fe 2+ levels in the BC cell lines were measured by an iron assay kit; E and F: qRT-PCR was performed to examine the mRNA levels of genes associated with ferroptosis. Each data point is expressed as mean ± SEM of 3–5 independent tests. **P<0.01, ***P<0.001. CUN, curcumin; ROS, reactive oxygen species; MDA, malondialdehyde; BC, breast cancer; qRT-PCR, quantitative real-time polymerase chain reaction; SEM, standard error of the mean.
Titel: |
CUN induces cell ferroptosis by upregulation of lipid peroxidation and iron accumulation.
|
---|---|
Autor/in / Beteiligte Person: | Xuelei Cao (8476722) ; Yao Li (154923) ; Yongbin Wang (849861) ; Tao Yu (176965) ; Chao Zhu (385240) ; Xuezhi Zhang (159645) ; Jialiang Guan (11965346) |
Link: | |
Veröffentlichung: | 2022 |
Medientyp: | Bild |
DOI: | 10.1371/journal.pone.0261370.g002 |
Schlagwort: |
|
Sonstiges: |
|