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International audience ; Several studies previously showed that the NFL-TBS.40-63 peptide (NFL-peptide) is capable to specifically penetrating several glioblastoma cell lines (rat, mouse, human) and inhibiting their cell division in vitro and their tumor development in vivo. When lipid nanocapsules (LNCs) are functionalized with the NFL-peptide, their absorption is targeted in glioblastoma cells both in vitro and in vivo. In the present study, we investigated the molecular architecture of these nanovectors (LNC-NFL) by using several microscopy techniques (transmission electron microscopy, cryo-electron microscopy, and cryo-electron tomography). We also used high-performance liquid chromatography (UPLC) technique to evaluate the interaction between LNCs and peptides. The work shows that the NFL-peptide forms stable long filaments along which the lipid nanocapsules interact strongly to form some sort of nanomolecular bracelets. This new construction composed of the NFL-peptide and lipid nanocapsules shows a better internalization in rat glioblastoma cells (F98 cells) than lipid nanocapsules alone.

Titel:	Characterization and quantification of the interaction between the NFL-TBS.40-63 peptide and lipid nanocapsules
Autor/in / Beteiligte Person:	Griveau, A ; Alnemeh-Al Ali, H ; Jourdain, M A ; Dupont, Aurelien ; Eyer, Joel ; Micro et Nanomédecines Translationnelles (MINT) ; Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) ; Centre de Microscopie de Rennes (MRic) ; Université de Rennes (UR)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) ; SFR UA 4208 Interactions Cellulaires et Applications Thérapeutiques (ICAT) ; Université d'Angers (UA) ; This work has been supported by “Ligue contre le Cancer 49“, and “Plan Cancer Inserm“to Joël Eyer. The salary of Audrey Griveau was covered by the “Plan Cancer Inserm“.
Link:	https://hal.science/hal-03800543 https://hal.science/hal-03800543/document Volltext https://doi.org/10.1016/j.ijpx.2022.100127
Zeitschrift:	ISSN: 2590-1567, 2022
Veröffentlichung:	HAL CCSD ; Elsevier, 2022
Medientyp:	academicJournal
DOI:	10.1016/j.ijpx.2022.100127
Schlagwort:	BIOT-NFL Biotinylated NFL-peptide BIOT-NFL-SCR Biotinylated-NFL-scrambled-peptides CEM Cryo-electron microscopy Cryo-ET Cryo-electron tomography FAM-NFL NFL-peptide coupled to 5-carboxyfluorescein FAM-NFL-SCR 5-carboxyfluorescein-NFL-scrambled-peptides GBM Glioblastoma Interaction Internalization LNC-(DiD) Lipid nanocapsule loaded with DiD LNC-(DiD)-BIOT-NFL Lipid nanocapsule loaded with DiD functionalized with LNC-(DiD)-BIOT-SCR-NFL LNC-(DiD)-FAM-NFL LNC-(DiD)-FAM-SCR-NFL LNCs Lipid nanocapsules NFL-SCR-peptides NFL-scrambled peptides NFL-TBS.40–63 peptide NFL-peptide NFL-TBS.40-63
Sonstiges:	Nachgewiesen in: BASE Sprachen: English Collection: Université de Rennes 1: Publications scientifiques (HAL) Document Type: article in journal/newspaper Language: English Relation: info:eu-repo/semantics/altIdentifier/pmid/36177093; hal-03800543; https://hal.science/hal-03800543; https://hal.science/hal-03800543/document; https://hal.science/hal-03800543/file/griveau_2022_Characterization%20and%20quantification%20of%20the%20interaction%20between%20the.pdf; PUBMED: 36177093 Rights: info:eu-repo/semantics/OpenAccess

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Characterization and quantification of the interaction between the NFL-TBS.40-63 peptide and lipid nanocapsules