Cryoprotective effect of glycerol concentrations on Indian Red Jungle Fowl (Gallus gallus murghi) spermatozoa
In: ISSN: 1758-1559, 2018
academicJournal
Zugriff:
International audience ; Semen cryopreservation protocols for wild avian species need to be optimised in order to achieve optimum post-thaw sperm quality and fertility. The present study was designed to evaluate the cryoprotective effect of different glycerol concentrations (11%, 15% and 20%) on post-thaw quality, recovery rates, absolute livability index and fertility of Indian Red Jungle Fowl (Gallus gallus murghi) semen. Semen was collected from eight mature cocks and cryopreserved for storage at -196 degrees C. Frozen semen was thawed at 37 degrees C for 30 s and assessed for motility, plasma membrane integrity, viability and acrosome integrity at 0, 2 and 4 h incubation at 37 degrees C. Percentages of motility, plasma membrane integrity, viability and acrosome integrity were recorded higher (P<0.05) post-thaw at 0, 2 and 4 h at 37 degrees C with 20% glycerol compared to 15% and 11% glycerol. Likewise, recovery rates (%) of aforementioned parameters after cryopreservation and absolute livability index were observed highest (P<0.05) with 20% glycerol. By comparing values of R-2 after multivariate regression analysis, least negative effects of hours of incubation were observed on semen quality in extenders with 20% glycerol followed by 15% and 11% glycerol. The fertility outcomes (number of fertile eggs, fertility [%], number of hatched chicks, percent hatch and hatchability of fertilised eggs) were recorded higher (P<0.05) with 20% glycerol followed by 15% and 11% glycerol. It is concluded that the concentration of 20% glycerol gives the best cryoprotection for quality and fertility of Indian Red Jungle Fowl semen.
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Cryoprotective effect of glycerol concentrations on Indian Red Jungle Fowl (Gallus gallus murghi) spermatozoa
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Autor/in / Beteiligte Person: | Rakha, Allah ; Ansari, Muhammad Sajjad ; Akhter, Shamim ; Blesbois, Elisabeth ; Pir Mehr Ali Shah Arid Agriculture University ; Sragodha, University ; Partenaires, INRAE ; Department de zoology ; The University of Hong Kong (HKU) ; Physiologie de la reproduction et des comportements Nouzilly (PRC) ; Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation Saumur -Université de Tours-Centre National de la Recherche Scientifique (CNRS) |
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Zeitschrift: | ISSN: 1758-1559, 2018 |
Veröffentlichung: | HAL CCSD ; SAGE Publications, 2018 |
Medientyp: | academicJournal |
DOI: | 10.3184/175815618X15180876264262 |
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