The effects of low-dose IL-2 on Th17/Treg cell imbalance in primary biliary cholangitis mouse models ...
figshare, 2024
academicJournal
Zugriff:
Background/aims Primary biliary cholangitis (PBC) is a chronic cholestatic liver disease. The imbalance of Th17/Treg cells has been reported in PBC patients. Low-dose IL-2 can alleviate disease severity through modulating CD4 + T cell subsets in patients with autoimmune diseases. Hence, the present study aimed to examine the effects and mechanism of low-dose IL-2 in PBC mouse models. Methods PBC models were induced in female C57BL/6 mice by two immunizations with 2OA-BSA at two-week intervals, and poly I: C every three days. PBC mouse models were divided into the IL-2 treated and untreated groups and low-dose IL-2 was injected at three different time points. Th17 and Tregs were analyzed by flow cytometry, and the related cytokines were analyzed by ELISA. Liver histopathology was examined by H&E and immunohistochemical staining. Results Twelve weeks after modeling, the serum AMA was positive and the ALP was significantly increased in PBC mouse models (P<0.05). The pathology showed lymphocyte ...
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The effects of low-dose IL-2 on Th17/Treg cell imbalance in primary biliary cholangitis mouse models ...
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Autor/in / Beteiligte Person: | Wang, Zilong ; Liu, Zhicheng ; Zheng, Jiarui ; Huang, Linxiang ; Jin, Rui ; Wang, Xiaoxiao ; Chen, Dongbo ; Xie, Yandi ; Feng, Bo |
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Veröffentlichung: | figshare, 2024 |
Medientyp: | academicJournal |
DOI: | 10.6084/m9.figshare.c.7093171 |
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