Improved reference genome of the arboviral vector Aedes albopictus
In: ISSN: 1465-6906, 2020
Online
academicJournal
Zugriff:
International audience ; Background: The Asian tiger mosquito Aedes albopictus is globally expanding and has become the main vector for human arboviruses in Europe. With limited antiviral drugs and vaccines available, vector control is the primary approach to prevent mosquito-borne diseases. A reliable and accurate DNA sequence of the Ae. albopictus genome is essential to develop new approaches that involve genetic manipulation of mosquitoes. Results: We use long-read sequencing methods and modern scaffolding techniques (PacBio, 10X, and Hi-C) to produce AalbF2, a dramatically improved assembly of the Ae. albopictus genome. AalbF2 reveals widespread viral insertions, novel microRNAs and piRNA clusters, the sex-determining locus, and new immunity genes, and enables genome-wide studies of geographically diverse Ae. albopictus populations and analyses of the developmental and stage-dependent network of expression data. Additionally, we build the first physical map for this species with 75% of the assembled genome anchored to the chromosomes. Conclusion: The AalbF2 genome assembly represents the most up-to-date collective knowledge of the Ae. albopictus genome. These resources represent a foundation to improve understanding of the adaptation potential and the epidemiological relevance of this species and foster the development of innovative control measures.
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Improved reference genome of the arboviral vector Aedes albopictus
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| Autor/in / Beteiligte Person: | Palatini, Umberto ; Masri, Reem, A ; Cosme, Luciano, V ; Koren, Sergey ; Thibaud-Nissen, Francoise ; Biedler, James, K ; Krsticevic, Flavia ; Johnston, J. Spencer ; Halbach, Rebecca ; Crawford, Jacob, E ; Antoshechkin, Igor ; Failloux, Anna-Bella ; Pischedda, Elisa ; Marconcini, Michele ; Ghurye, Jay ; Rhie, Arang ; Sharma, Atashi ; Karagodin, Dmitry, A ; Jenrette, Jeremy ; Gamez, Stephanie ; Miesen, Pascal ; Masterson, Patrick ; Caccone, Adalgisa ; Sharakhova, Maria, V ; Tu, Zhijian ; Papathanos, Philippos, A ; van Rij, Ronald, P ; Akbari, Omar, S ; Powell, Jeffrey ; Phillippy, Adam, M ; Bonizzoni, Mariangela ; Dipartimento di Biologia e Biotecnologie "Lazzaro Spallanzani" = Department of Biology and Biotechnology Univ di Pavia (DBB UNIPV) ; Università degli Studi di Pavia = University of Pavia (UNIPV) ; Virginia Polytechnic Institute and State University Blacksburg ; Department of Ecology and Evolutionary Biology New Haven ; Yale University New Haven ; National Human Genome Research Institute (NHGRI) ; National Center for Biotechnology Information (NCBI) ; National Institutes of Health Bethesda (NIH) ; The Hebrew University of Jerusalem (HUJ) ; Texas A&M University College Station ; Radboud University Medical Center Nijmegen ; Verily Life Sciences Inc ; California Institute of Technology (CALTECH) ; Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors ; Institut Pasteur Paris (IP) ; Siberian Branch of the Russian Academy of Sciences (SB RAS) ; University of California San Diego (UC San Diego) ; University of California (UC) ; Tomsk State University Tomsk ; National Biodefense Analysis and Countermeasures Center Frederick ; U.S. Social Security Administration ; The authors would like to thank the following for their financial support of research: European Research Council (ERC-CoG 682394 to M. Bonizzoni ; ERC-CoG 615680 to R.P. van Rij) ; Italian Ministry of Education, University and Research (FARE-MIUR project R1623HZAH5 to M. Bonizzoni and Dipartimenti Eccellenza Program 2018–2022 to Department of Biology and Biotechnology “L. Spallanzani,” University of Pavia) ; Human Frontiers Science Foundation (Research Grant number RGP0007/2017) to M. Bonizzoni and R.P. van Rij ; National Institutes of Health (R21AI135258 to M. Sharakova ; 1R01AI151004-01 and 1DP2AI152071-01 to O.S. Akbari ; R01AI32409 to A. Caccone) ; Defense Advanced Research Project Agency (DARPA) Safe Genes Program Grant (HR0011-17-2-0047) to O.S. Akbari ; Netherlands Organization for Scientific Research (VICI grant number 016.VICI.170.090) to R.P. van Rij ; Israeli Ministry of Science and Technology (3-16795 to P.A. Papathanos) ; Intramural Research Program of the National Human Genome Research Institute, National Institutes of Health to J. Ghurye, A. Rhie, and A.M. Phillippy ; Intramural Research Program of the National Library of Medicine, National Institutes of Health to F. Thibaud-Niessen ; generous UCSD lab. startup funds to O.S. Akbari. ; We would like to thank Francesca Scolari and Patrizia Chiari for the mosquito maintenance. ; European Project: 682394,H2020,ERC-2015-CoG,NIRV_HOST_INT(2016) ; European Project: 615680,EC:FP7:ERC,ERC-2013-CoG,VIVARNASILENCING(2014) |
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| Zeitschrift: | ISSN: 1465-6906, 2020 |
| Veröffentlichung: | HAL CCSD ; BioMed Central, 2020 |
| Medientyp: | academicJournal |
| DOI: | 10.1186/s13059-020-02141-w |
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