A new, multiplex, quantitative real-time polymerase chain reaction system for nucleic acid detection and quantification
Humana Press, 2013
Buch
Zugriff:
Quantitative real-time polymerase chain reaction (qPCR) has emerged as a powerful investigative and diagnostic tool with potential to generate accurate and reproducible results. qPCR can be designed to fulfil the four key aspects required for the detection of nucleic acids: simplicity, speed, sensitivity, and specificity. This chapter reports the development of a novel real-time multiplex quantitative PCR technology, dubbed PrimRglo (TM), with a potential for high-degree multiplexing. It combines the capacity to simultaneously detect many viruses, bacteria, or nucleic acids, in a single reaction tube, with the ability to quantitate viral or bacterial load. The system utilizes oligonucleotide-tagged PCR primers, along with complementary fluorophore-labelled and quencher-labelled oligonucleotides. The analytic sensitivity of PrimRglo technology was compared with the widely used Taqman (R) and SYBR green detection systems.
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A new, multiplex, quantitative real-time polymerase chain reaction system for nucleic acid detection and quantification
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Autor/in / Beteiligte Person: | Liang, Fang ; Arora, Neetika ; Zhang, Kang Liang ; Yeh, David Che Cheng ; Lai, Richard ; Pearson, Darnley ; Barnett, Graeme ; Whiley, David ; Sloots, Theo ; Corrie, Simon R. ; Barnard, Ross T. ; Kolpashchikov, Dmitry M. ; Gerasimova, Yulia V. |
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Veröffentlichung: | Humana Press, 2013 |
Medientyp: | Buch |
ISSN: | 1064-3745 (print) ; 1940-6029 (print) |
DOI: | 10.1007/978-1-62703-535-4_4 |
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