P. aeruginosa CF27 biofilm dispersal at 0, 3, 6, 12, and 24 hours after treatment with, buffer control (non-treated), or 16 μg/mL tobramycin (TOB) individually or with 250 U/mL AlyP1400 (AlyP1400+TOB).

Sequential treatment (AlyP1400→TOB) data were only acquired for 0 h time point, therefore were not included in this figure. (A) Live events per millilitre (events/mL) as determined using flow cytometric analysis of the cell populations for living cells (events negative for FVS520 and positive for FVS700). (B) The total viable count (CFU/mL) of P . aeruginosa CF27 living dispersed biofilm cells. (C) Dead events per millilitre (events/mL) as determined using flow cytometric analysis of the dead populations (events positive for both FVS520 and FVS700). Statistical significance was determined using ANOVA two-way analysis with Tukey’s test multiple comparison test. * P < 0.05, ** P < 0.01 compared with non-treated control. The standard error of mean of three independent flow cells is indicated by the error bars. (TIF)