New strategies for detailed visualisation and analysis of stem cell-material interactions
Imperial College London, 2018
Online
Hochschulschrift
Zugriff:
The success of stem cell therapy and regenerative medicine relies on providing bio- chemical and biophysical cues to safely and reliably control cell fate transitions. It has now been recognised that recapitulating the physical attributes of the stem cell microenvironment is a key feature in controlling cell behaviour. Of particular interest is the ability of the extracellular microenvironment to present surface topographical features that contribute to control of cell attachment and morphology, which have a direct impact on maintenance of cell phenotype and gene expression. This realisation has led to the development of materials with surface topography to probe and control the stem cell behaviour. In this thesis, the influence of material topography on cell behaviour is investigated through the use of both microscale and nanoscale topography, with a particular focus on visualisation strategies to characterise the features of the cell-material interface. Firstly, microscale topography in the form of microgrooves, is used to study the impact of topography at the level of the nucleus in neural stem cells. Through the development of new visualisation methods, incorporating state-of-the-art focused-ion beam scanning electron microscopy and scanning ion conductance microscopy, the nuclei of cells cul- tured on microgrooved topography are shown to correlatively remodel their nuclear architecture, epigenetics and mechanical properties. Secondly, using nanoscale topography in the form of high aspect ratio nanoneedle arrays, the cell-material interface is investigated at the level of the plasma membrane in human mesenchymal stem cells. Nanoneedles, typically used to deliver a variety of therapeutically relevant payloads, are shown to create a tight interface with the cell membrane, while triggering changes in plasma membrane architecture and inducing the activation of endocytosis pathways, which contributes significantly to payload delivery at the interface.
Titel: |
New strategies for detailed visualisation and analysis of stem cell-material interactions
|
---|---|
Autor/in / Beteiligte Person: | Gopal, Sahana ; Korchev, Yuri ; Stevens, Molly ; Shevchuk, Andrew |
Link: | |
Veröffentlichung: | Imperial College London, 2018 |
Medientyp: | Hochschulschrift |
DOI: | 10.25560/83028 |
Schlagwort: |
|
Sonstiges: |
|