Electrochemical and Raman spectroscopic studies of the effect of lanthanide ions on the activity of mitochondrial malate dehydrogenase
In: Journal of electroanalytical chemistry (1992), Jg. 499 (2001), Heft 1, S. 24-29
academicJournal
- print, 25 ref
Zugriff:
Chronoamperometry based on the 'diffusion' layer concept of the convective system was used to assay the activity of mitochondrial malate dehydrogenase (MDH). When the enzyme-catalysed reaction was initiated by adding the enzyme MDH into a well-stirred nicotinamide adenine dinucleotide (NADH, coenzyme) solution, the enzyme activity could be indicated by the continuous in-situ decrease in the limiting steady-state oxidation current of NADH. The effects of some lanthanide ions on the MDH activity were monitored. The La3+, Ce3+ and Eu3 ions could activate markedly the enzyme MDH as the concentrations were lower. The activation mechanism would be that the lanthanide ions could interrupt the binding of NAD+ to MDH by combining preferentially to NAD+. This mechanism was proposed on the basis of voltammetric and Raman spectroscopic studies.
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Electrochemical and Raman spectroscopic studies of the effect of lanthanide ions on the activity of mitochondrial malate dehydrogenase
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Autor/in / Beteiligte Person: | ZHUANG, Qian-Kun ; DAI, Huai-Cheng |
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Zeitschrift: | Journal of electroanalytical chemistry (1992), Jg. 499 (2001), Heft 1, S. 24-29 |
Veröffentlichung: | Amsterdam: Elsevier Science, 2001 |
Medientyp: | academicJournal |
Umfang: | print, 25 ref |
ISSN: | 1572-6657 (print) |
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