Comprehensive two dimensional separation based on coupling micellar electrokinetic chromatography with capillary isoelectric focusing

LI, SHENG ; PAWLISZYN, Janusz

In: Analyst (London. 1877. Print), Jg. 127 (2002), Heft 9, S. 1159-1163

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Concentrating properties of the Capillary Isoelectric Focusing (CIEFj system with continuous whole-column-imaging detection were investigated for application as a second dimension in a comprehensive two-dimensional (2D) separation process. The concentration/separation/detection was completed within 4 min in a 300 μm inner diameter capillary. As the key to the successful coupling of CIEF to a first dimension separation, a novel interface was developed. A 10-port valve with two conditioning loops was used to perform both comprehensive collection and dialysis desalting of the first dimensional effluent, and as an interface coupling Micellar Electrokinetic Chromatography (MEKC) to CIEF. In the loop, salt and other unwanted first dimension effluent components were eliminated by dialysis and carrier ampholytes (CAs) were added. Peak broadening during the dialysis did not have significant impact on the CIEF separation because of its concentrating effect. Protein digests were first separated by MEKC followed by isoelectric point (pI) using whole-column-imaged CIEF. The dialysis interface allows general coupling of the whole-column-imaged CIEF to microscale separations.

Titel:	Comprehensive two dimensional separation based on coupling micellar electrokinetic chromatography with capillary isoelectric focusing
Autor/in / Beteiligte Person:	LI, SHENG ; PAWLISZYN, Janusz
Link:	Full Text Finder (Volltext) View record from PASCAL Archive
Zeitschrift:	Analyst (London. 1877. Print), Jg. 127 (2002), Heft 9, S. 1159-1163
Veröffentlichung:	Cambridge: Royal Society of Chemistry, 2002
Medientyp:	academicJournal
Umfang:	print, 38 ref
ISSN:	0003-2654 (print)
Schlagwort:	Analytical chemistry Chimie analytique Sciences exactes et technologie Exact sciences and technology Chimie Chemistry Méthodes chromatographiques et méthodes physiques associées à la chromatographie Chromatographic methods and physical methods associated with chromatography Autres méthodes chromatographiques Other chromatographic methods Sciences biologiques et medicales Biological and medical sciences Sciences biologiques fondamentales et appliquees. Psychologie Fundamental and applied biological sciences. Psychology Biochimie analytique, structurale et metabolique Analytical, structural and metabolic biochemistry Biochimie analytique: généralités, techniques, appareillages Analytical biochemistry: general aspects, technics, instrumentation Chromatographie MECC Micellar electrokinetic capillary chromatography Cromatografía MECC Détecteur UV Ultraviolet detector Detector UV Electrophorèse bidimensionnelle Two dimensional electrophoresis Electrofóresis bidimensional Electrophorèse capillaire Capillary electrophoresis Electroforesis capilar Evaluation performance Performance evaluation Evaluación prestación Focalisation isoélectrique Isoelectric focusing Focalización isoeléctrica Macromolécule biologique Biological macromolecule Macromolécula biológica Méthode analyse Analysis method Método análisis Méthode séparation Separation method Método separación
Sonstiges:	Nachgewiesen in: PASCAL Archive Sprachen: English Original Material: INIST-CNRS Document Type: Article File Description: text Language: English Author Affiliations: Department of Chemistry, University of Waterloo, Waterloo, ON, N2L 3G1, Canada Rights: Copyright 2002 INIST-CNRS ; CC BY 4.0 ; Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS Notes: Analytical chemistry ; Analytical, structural and metabolic biochemistry

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