Subcellular localization and isoenzyme pattern of peroxidase and polyphenol oxidase in beet root (Beta vulgaris L.)

ESCRIBANO, Josefa ; GANDIA-HERRERO, Fernando ; et al.

In: Journal of agricultural and food chemistry (Print), Jg. 50 (2002), Heft 21, S. 6123-6129

academicJournal - print, 45 ref

The two enzymes involved in enzymatic browning reactions, polyphenol oxidase (PPO) and peroxidase (PO), have been partially purified and extracted from different fractions of beet root. PPO is mainly located in the membrane fraction, and it was also found in the soluble fraction. In both cases PPO was in its latent state. However, PO activity was higher in the soluble fraction than in the membrane fraction. Nevertheless, the highest values of specific activity for PO were obtained from the solubilized enzyme from acetone powders. Under native isoelectric focusing (IEF), several PPO isoenzymes were present in the pH range of 4.8-5.8. All of these isoenzymes shared a single band with a similar apparent mass under sodium dodecyl sulfate-polyacrylamide gel electrophoresis. PO was also analyzed by IEF, showing a complex isoenzyme pattern in all fractions. The characteristic basic PO isoenzyme of high pl found in both the soluble fraction and the solubilized enzyme from acetone powders was not detected in the membrane fraction. The kinetic characterization of PPO and PO from all fractions was carried out.

Titel:	Subcellular localization and isoenzyme pattern of peroxidase and polyphenol oxidase in beet root (Beta vulgaris L.)
Autor/in / Beteiligte Person:	ESCRIBANO, Josefa ; GANDIA-HERRERO, Fernando ; CABALLERO, Nuria ; PEDRENO, Maria Angeles
Link:	E-Journal im Bestand der UB Hagen? View record from PASCAL Archive
Zeitschrift:	Journal of agricultural and food chemistry (Print), Jg. 50 (2002), Heft 21, S. 6123-6129
Veröffentlichung:	Washington, DC: American Chemical Society, 2002
Medientyp:	academicJournal
Umfang:	print, 45 ref
ISSN:	0021-8561 (print)
Schlagwort:	Agronomy, agriculture, phytopathology Agronomie, agriculture, phytopathologie Food science technology Sciences technologies alimentaires Sciences biologiques et medicales Biological and medical sciences Sciences biologiques fondamentales et appliquees. Psychologie Fundamental and applied biological sciences. Psychology Agronomie. Sciences du sol et productions vegetales Agronomy. Soil science and plant productions Physiologie des plantes d'intérêt économique Economic plant physiology Nutrition. Photosynthèse. Respiration. Métabolisme Nutrition. Photosynthesis. Respiration. Metabolism Enzymes Industries agroalimentaires Food industries Industries des fruits et légumes Fruit and vegetable industries Angiospermae Chenopodiaceae Dicotyledones Enzyme Enzima Oxidoreductases Peroxidases Plante Plant Planta Spermatophyta Activité enzymatique Enzymatic activity Actividad enzimática Activité spécifique Specific activity Actividad específica Beta vulgaris Brunissement enzymatique Enzymic browning Bronceado enzimático Catechol oxidase Compartiment cellulaire Cell compartment Compartimiento celular Détection Detection Detección Forme soluble Soluble form Forma soluble Isozyme Isozima Localisation Localization Localización Membrane biologique Biomembrane Membrana biológica Peroxidase Physiologie après récolte Postharvest physiology Fisiología después cosecha Phénol Phenol Fenol Purification Purificación Racine Root Raíz Beta vulgaris L isoenzyme pattern polyphenol oxidase
Sonstiges:	Nachgewiesen in: PASCAL Archive Sprachen: English Original Material: INIST-CNRS Document Type: Article File Description: text Language: English Author Affiliations: Departamento de Bioquímica y Biología Molecular A, Unidad Docente de Biología, Facultad de Veterinaria, Universidad de Murcia, 30100 Espinardo, Murcia, Spain Rights: Copyright 2003 INIST-CNRS ; CC BY 4.0 ; Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS Notes: Agrifood industries ; Agronomy. Soil sciences and vegetal productions

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