Effects of UVA and L-ascorbic acid on nuclear factor-kB in melanocytes and in HaCaT keratinocytes
In: Free radicals and skin: Basic research, aging, photocarcinogenesis. 4th Teupitzer colloquium, September 28 to October 1, 2001Skin pharmacology and applied skin physiology 15(5):353-359; Jg. 15 (2002) 5, S. 353-359
Konferenz
- print, 20 ref
Zugriff:
Nuclear factor-KB (NFκB) is a pleiotropic tran-scriptional activator, which is a sensitive transcriptional factor for free radicals and activates multiple target genes. UVA is very efficient in inducing free radicals in human skin cells. L-ascorbic acid is regarded as a scavenger of UVA-induced free radicals in human keratinocytes. In epidermis, melanocytes and keratinocytes play an important protective role against skin photodamage. In the present study, we aimed to investigate the role of NFKB on photodamage in melanocytes and keratinocytes. Normal human melanocytes (NHM) and HaCaT keratinocytes were treated with UVA (500 mJ/cm2, 1,000 mJ/cm2) and/or L-ascorbic acid (100 pM, 250 μM). NFKB binding activity was analysed by electrophoretic mobility shift assay. NFKB binding activity was increased by UVA irradiation in HaCaT keratinocytes, but it was not affected in NHM. On the other hand, L-ascorbic acid decreased NFKB binding activity both in UVA-irradiated and in non-irradiated NHM. In contrast, NFKB binding activity in HaCaT keratinocytes was increased after treatment with L-ascorbic acid. In addition, L-ascorbic acid synergistically induced NFKB binding activity with UVA irradiation. The contrary response on NFKB binding activity in NHM and HaCaT keratinocytes indicated that the redox regulation might be different on photoprotective action in melanocytes and keratinocytes.
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Effects of UVA and L-ascorbic acid on nuclear factor-kB in melanocytes and in HaCaT keratinocytes
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Autor/in / Beteiligte Person: | JING, SHANG ; SCHWARZ, C ; SANCHEZ RUDERISCH, H ; HERTTING, T ; ORFANOS, C. E ; TEBBE, B |
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Quelle: | Free radicals and skin: Basic research, aging, photocarcinogenesis. 4th Teupitzer colloquium, September 28 to October 1, 2001Skin pharmacology and applied skin physiology 15(5):353-359; Jg. 15 (2002) 5, S. 353-359 |
Veröffentlichung: | Basel: Karger, 2002 |
Medientyp: | Konferenz |
Umfang: | print, 20 ref |
ISSN: | 1422-2868 (print) |
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