Identification and synthesis of a male-produced sex pheromone from the stink bug Chlorochroa sayi

The reproductive behavior of the stink bug Chlorochroa sayi was studied in the laboratory. There was a sexual maturation period of about 10 days before hugs began mating. Sexually mature adult hugs engaged in courtship consisting of antennation and head-butting of the female by the male, before the female adopted a receptive posture and copulation occurred Both sexes mated multiple times during their life-spans, with the mean duration of copulations of virgin bugs (42.3 ± 19.6 min) and experienced bugs (37.3 ± 28.4 min) being similar. Most matings were initiated in the late afternoon or evening, when pheromone production by males was greatest. Males transferred sperm and nutrients constituting about 17% of their body weight to females during mating. Three male-specific components, methyl geranate. methyl citronellate, and methyl (E)-6-2,3-dihydrofarnesoate in a ratio of 100: 0.45: 1.6, were first detected in volatiles collected from male bugs on green beans about 9-12 2 days after the final molt to the adult stage. In vertical Y-tube bioassays, females were attracted to odors from mature male bugs and to a blend of the three male-produced components. Low numbers of females also were attracted in field trials with the three-component blend. The relatively weak attraction may he a result of other, as yet unknown cues being required in addition to the pheromone such as visual or substrate-borne vibrational cues.