Kinetics of CFU-Mk after automated plateletpheresis
In: Vox sanguinis (Basel. 1956), Jg. 81 (2001), Heft 3, S. 167-171
Online
academicJournal
- print, 12 ref
Zugriff:
Background and Objectives Platelet count, thrombopoetin (TPO) level and the compartment of megakaryocyte progenitor cells (CFU-Mk) are major determinants in the regulation of thrombopoiesis. The aim of this study was to investigate the potential changes in the compartment of CFU-Mk and their correlation with serum TPO levels and platelet count after plateletpheresis. Materials and methods Twelve healthy individuals were randomly assigned to undergo single-donor plateletpheresis. A collagen-based in vitro culture system was used to determine the number of peripheral blood (PB) CFU-Mk before and after donation and on days 1, 4 and 7 thereafter. TPO levels were measured by a specific enzyme-linked immunosorbent assay and whole blood counts were performed using an automated cell counter. Results The pre-apheresis platelet count (mean ± SEM: 276 ± 13 x 109/l) decreased after plateletpheresis to a nadir of 194 ± 8 x 109/l (P < 0.001), showed a gradual increase on days 1 and 4, and reached pre-apheresis values by day 7 (280 ± 11). The serum TPO levels were found to be significantly increased on days 1 and 7 as compared to baseline levels (baseline value 103.3 ± 18.5 pg/ml versus day-1 value 135.8 ± 25.8 pg/ml and day-7 value 132 ± 30.19 pg/ml; P < 0.03 and P < 0.03, respectively). The numbers of CFU-Mk in PB were significantly elevated on day 4 only (125 ± 21 colonies/ml of PB versus pre-apheresis values of 68 ± 18 colonies/ml of PB, P < 0.005). Conclusions These findings suggest that platelet loss during plateletpheresis affects thrombopoiesis at the progenitor cell level, probably through alterations in TPO plasma concentrations.
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Kinetics of CFU-Mk after automated plateletpheresis
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Autor/in / Beteiligte Person: | WAGNER, T ; SCHWARTZ, D. W. M ; WINTER, M ; KABRNA, E ; KOLLARS, M ; SCHWARZINGER, I ; LANZER, G ; MAYR, W. R ; GEISSLER, K |
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Zeitschrift: | Vox sanguinis (Basel. 1956), Jg. 81 (2001), Heft 3, S. 167-171 |
Veröffentlichung: | Oxford: Blackwell, 2001 |
Medientyp: | academicJournal |
Umfang: | print, 12 ref |
ISSN: | 0042-9007 (print) |
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