HIV-1 Vpr binding to HIV-1 LTR C/EBP cis-acting elements and adjacent regions is sequence-specific
In: AIDS, Jg. 57 (2003), Heft 1, S. 41-48
academicJournal
- print, 31 ref
Zugriff:
Human immunodeficiency virus type 1 (HIV-1) viral protein R (Vpr) is a 14 kDa virion-associated protein that transactivates the HIV-1 long terminal repeat (LTR) as well as other eukaryotic promoters. Vpr also functions in nuclear localization and import of the HIV-1 preintegration complex (PIC), cell cycle arrest at the G2/M interface, and virion packaging. Electrophoretic mobility shift analysis has been utilized to demonstrate a direct association between purified Vpr (strain pNL4-3) and HIV-1 LTR sequences that span the adjacent C/EBP site I, NF-KB site II, and ATF/CREB binding site (nt -95 to -130, relative to the start of transcription). A similar interfaction has been observed between HIV-1 Vpr and LTR C/EBP site II (nt -167 to -175). A total of 94.7% of LTRs derived from peripheral blood contained C/EBP site I variants that displayed a high relative Vpr binding affinity phenotype, while only 5.3% exhibited a low relative Vpr binding affinity phenotype. All LTRs derived from peripheral blood exhibited a high relative Vpr binding phenotype at C/EBP site 11. These results suggest a preference for the maintenance of two cis-acting elements with high affinity for Vpr within LTRs derived from peripheral blood. Additional studies have also demonstrated that naturally occurring sequence variation within C/EBP site I and II can dramatically alter the relative affinity of Vpr for these cis-acting elements. These studies suggest that Vpr may regulate the interaction of members of the C/EBP transcription factor family with the viral LTR.
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HIV-1 Vpr binding to HIV-1 LTR C/EBP cis-acting elements and adjacent regions is sequence-specific
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Autor/in / Beteiligte Person: | HOGAN, Tricia H ; NONNEMACHER, Michael R ; KREBS, Fred C ; HENDERSON, Andrew ; WIGDAHL, Brian |
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Zeitschrift: | AIDS, Jg. 57 (2003), Heft 1, S. 41-48 |
Veröffentlichung: | Paris: Elsevier, 2003 |
Medientyp: | academicJournal |
Umfang: | print, 31 ref |
ISSN: | 0753-3322 (print) |
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