Purification and characterization of an NAD-dependent malate dehydrogenase from leaves of the crassulacean acid metabolism plant Aptenia cordifolia

TRINODI, Karina E. J ; PODESTA, Florencio E

In: Plant physiology and biochemistry (Paris), Jg. 41 (2003), Heft 2, S. 97-102

academicJournal - print, 35 ref

An NAD-malate dehydrogenase (NAD-MDH, EC 1.1.1.37) was purified and characterized from leaves of Aptenia cordifolia L. f. (Schwant). This plant performs crassulacean acid metabolism (CAM), as indicated by: (a) elevated levels of phosphoenolpyruvate carboxylase (PEPC) and NAD(P) malic enzyme; (b) regulation of PEPC compatible with its function during the night; (c) characteristic day/night changes in titratable acidity; and (d) gas exchange profile consistent with that shown by CAM plants. These features remained unchanged by water availability or salt stress, suggesting constitulive CAM. The purified MDH showed a subunit molecular mass of 39.4 kDa, a native mass of 83 kDa (dimer) and a pI of 5.8. It cross-reacted with antibodies against cytosolic malate dehydrogenase (cMDH) from pineapple. Maximum activities for oxaloacetate (OAA) reduction or malate oxidation were observed at pH 7.0 and between pH 7.2 and 8.4, respectively. The enzyme was inhibited by excess OAA, in a pH-dependent manner. A discontinuity was observed in Arrhenius plots at 33 °C, with an activation energy twice as high below this temperature. Although immunologically related, some physical and kinetic dissimilarities between the A. cordifolia and pineapple enzymes suggest that diverse CAM metabolic subtypes may require different MDH isozymes to carry out OAA reduction.

Titel:	Purification and characterization of an NAD-dependent malate dehydrogenase from leaves of the crassulacean acid metabolism plant Aptenia cordifolia
Autor/in / Beteiligte Person:	TRINODI, Karina E. J ; PODESTA, Florencio E
Link:	E-Journal im Bestand der UB Hagen? View record from PASCAL Archive
Zeitschrift:	Plant physiology and biochemistry (Paris), Jg. 41 (2003), Heft 2, S. 97-102
Veröffentlichung:	Paris: Elsevier, 2003
Medientyp:	academicJournal
Umfang:	print, 35 ref
ISSN:	0981-9428 (print)
Schlagwort:	Agronomy, agriculture, phytopathology Agronomie, agriculture, phytopathologie Biochemistry, molecular biology, biophysics Biochimie, biologie moléculaire, biophysique Cell biology, histology Biologie cellulaire, histologie Plant biology and physiology Biologie et physiologie végétales Biotechnology Biotechnologies Sciences biologiques et medicales Biological and medical sciences Sciences biologiques fondamentales et appliquees. Psychologie Fundamental and applied biological sciences. Psychology Physiologie vegetale et developpement Plant physiology and development Métabolisme Metabolism Enzymes Aizoaceae Angiospermae Dicotyledones Enzyme Enzima Oxidoreductases Spermatophyta Activité enzymatique Enzymatic activity Actividad enzimática Cinétique Kinetics Cinética Feuille végétal Plant leaf Hoja vegetal Localisation Localization Localización Malate dehydrogenase Photosynthèse Photosynthesis Fotosíntesis Purification Purificación Type CAM CAM-Type Tipo CAM Aptenia cordifolia CAM Enzyme regulation Intracellular localization MDH
Sonstiges:	Nachgewiesen in: PASCAL Archive Sprachen: English Original Material: INIST-CNRS Document Type: Article File Description: text Language: English Author Affiliations: CEFOBI, Facultad de Ciencias Bioquímicas y Farmacéuticas, Suipacha 531, 2000 Rosario, Argentina Rights: Copyright 2003 INIST-CNRS ; CC BY 4.0 ; Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS Notes: Cytology, morphology, systematics, floristics, plants physiology, evolution and development

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