High frequency of DAP-kinase gene promoter methylation in colorectal cancer specimens and its identification in serum
In: Cancer letters, Jg. 194 (2003), Heft 1, S. 99-105
academicJournal
- print, 32 ref
Zugriff:
Death-associated protein (DAP)-kinase is frequently inactivated by promoter methylation in human cancers. To understand the involvement of the DAP-kinase gene in colorectal cancer (CRC), we investigated the methylation of the DAP-kinasegene in primary CRC to define the frequency of this epigenetic aberration and the clinicopathological significance. For this reason, methylation-specific polymerase chain reaction (MSP) was used to detect DAP-kinase gene methylation in DNA from 122 cases of CRC and 18 paired serum samples. Methylation of the DAP-kinase gene was found in 67 of 122 (55%) cases of primary CRC. Study of the serum DNA from 14 patients exhibiting methylated DAP-kinase gene revealed aberrant methylation in three patients (21%). False positives were not obtained in any of the patients who did not exhibit methylation. No association was found between the promoter methylation of the DAP-kinase gene in primary CRC and gender, localization, tumor differentiation, invasion depth, regional lymph node involvement, or tumor stage. In conclusion, methylation of the DAP-kinase gene is common in CRC. The detection of the methylation of the DAP-kinase gene has a potential clinical application as a diagnostic tumor marker for CRC.
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High frequency of DAP-kinase gene promoter methylation in colorectal cancer specimens and its identification in serum
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Autor/in / Beteiligte Person: | YAMAGUCHI, Satoru ; ASAO, Takayuki ; NAKAMURA, Jun-Ichi ; IDE, Munenori ; KUWANO, Hiroyuki |
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Zeitschrift: | Cancer letters, Jg. 194 (2003), Heft 1, S. 99-105 |
Veröffentlichung: | Shannon: Elsevier, 2003 |
Medientyp: | academicJournal |
Umfang: | print, 32 ref |
ISSN: | 0304-3835 (print) |
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