The differential effects of cyclophosphamide, epirubicin and 5-fluorouracil on apoptotic marker (CPP-32), pro-apoptotic protein (p21WAF-1) and anti-apoptotic protein (bcl-2) in breast cancer cells
In: Breast cancer research and treatment, Jg. 80 (2003), Heft 3, S. 239-244
Online
academicJournal
- print, 22 ref
Zugriff:
Cyclophosphamide (CYC), epirubicin (EPI) and 5-fluorouracil (5FU) are commonly used cytotoxic drugs for the treatment of breast cancer. The efficacy of these drugs in the induction of caspases (CPP-32), pro-apoptotic (p21WAF-1) and anti-apoptotic (bcl-2) proteins is tested in vitro on breast cancer cells lines MDA-MB-231 and MCF-7. The cell proliferation rate and the levels of CPP-32, p21WAF-1 and bcl-2 are measured at 3, 6, 12 and 24 h. For MDA-MB-231 all three drugs caused significant inhibition in cell growth. CYC produces significant induction of CPP-32 at 3-6 h for MCF-7 only. For MDA-MB-231 and MCF-7, respectively, EPI induces CPP-32 at significant levels at 12-24 h and 6-12 h while 5FU creates induction for MDA-MB-231 at 3 h and for MCF-7 at 3-12 h. The levels of expression of p21WAF-1 and bcl-2 for all test groups were significantly different from their respective control groups. In the case of MDA-MB-231, regression analysis reveals that changes in CPP-32 levels and p21WAF-1 levels have a significant positive relationship. In all likelihood, other mechanisms of cell death are implicated in the antitumor effect of these drugs, beyond the activation of CPP-32 and p21WAF-1 as described in this paper.
Titel: |
The differential effects of cyclophosphamide, epirubicin and 5-fluorouracil on apoptotic marker (CPP-32), pro-apoptotic protein (p21WAF-1) and anti-apoptotic protein (bcl-2) in breast cancer cells
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Autor/in / Beteiligte Person: | CHOW, Louis W. C ; LOO, Wings T. Y |
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Zeitschrift: | Breast cancer research and treatment, Jg. 80 (2003), Heft 3, S. 239-244 |
Veröffentlichung: | Dordrecht: Springer, 2003 |
Medientyp: | academicJournal |
Umfang: | print, 22 ref |
ISSN: | 0167-6806 (print) |
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