Na+ and the substrate permeation pathway in dopamine transporters
In: Transporters as targets for drugs and endogenous compounds, Jg. 479 (2003), Heft 1-3, S. 213-221
academicJournal
- print, 1 p.1/2
Zugriff:
Advances have been made in characterizing the relationship between Na+ and the substrate permeation pathway in the dopamine transporter. This review covers the role of Na in co-transport with dopamine as well as in the recognition of dopamine. Apparent recognition depends on the preparation studied: it differs between intact cells heterologously expressing the dopamine transporter and membranes prepared from these cells. In our search for amino acid residues in the transporter involved in Na+ action, W84 and D313 were found to play a special role in cation interaction, with evidence for regulation of both Na+ and H+ sensitivity. Mutation of D313 to N appeared to decrease the affinity for the dopamine transporter in intact cells, not by altering recognition per se. A model is proposed in which access of dopamine, not recognition itself, is regulated by D313 and Na+. Thus, the role of external Na+ in intact cell preparations is to turn dopamine transporters to the externally facing form, allowing access of dopamine to its binding site.
Titel: |
Na+ and the substrate permeation pathway in dopamine transporters
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Autor/in / Beteiligte Person: | NIANHANG, CHEN ; REITH, Maarten E. A |
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Zeitschrift: | Transporters as targets for drugs and endogenous compounds, Jg. 479 (2003), Heft 1-3, S. 213-221 |
Veröffentlichung: | Amsterdam: Elsevier, 2003 |
Medientyp: | academicJournal |
Umfang: | print, 1 p.1/2 |
ISSN: | 0014-2999 (print) |
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