Molecular cloning of a rat brain cDNA, with homology to a tyrosine kinase substrate, that induces galactosylceramide expression in COS-7 cells
In: Journal of neurochemistry, Jg. 71 (1998), Heft 5, S. 1827-1836
Online
academicJournal
- print, 39 ref
Zugriff:
A rat brain cDNA clone has been isolated, using a eukaryotic cell transient expression system in conjunction with an anti-galactosylceramide (anti-GalCer) monoclonal antibody that induces GalCer expression in COS-7 cells. The protein was designated as GalCer expression factor-1 (GEF-1). A good correlation between GalCer expression and the level of the enzyme activity of UDP-galactose:ceramide galactosyltransferase (CGT) was demonstrated. The cDNA insert encoded a polypeptide of 771 amino acids with a calculated molecular mass of 85,787 Da. The cDNA hybridized to a single mRNA of 3.1 kb in all rat organs examined, including brain, testis, and skeletal muscle. The cDNA product was determined to be a tyrosine-phosphorylated protein with a molecular mass of 110 kDa in transfected COS-7 cells and adult rat brain. COS-7 cells transfected with the cDNA clone showed dramatic morphological changes: The transfected cells appeared to be fibroblast-like cells, whereas the parent COS-7 cells were typical epithelial-like cells. The deduced amino acid sequences revealed a strikingly high homology to a mouse hepatocyte growth factor-regulated tyrosine kinase substrate but no homology to CGT. Taking these results together, it is suggested that GEF-1 may play an important role in regulating GalCer expression in the brain.
Titel: |
Molecular cloning of a rat brain cDNA, with homology to a tyrosine kinase substrate, that induces galactosylceramide expression in COS-7 cells
|
---|---|
Autor/in / Beteiligte Person: | OGURA, K ; KOHNO, K ; TAI, T |
Link: | |
Zeitschrift: | Journal of neurochemistry, Jg. 71 (1998), Heft 5, S. 1827-1836 |
Veröffentlichung: | Oxford: Blackwell, 1998 |
Medientyp: | academicJournal |
Umfang: | print, 39 ref |
ISSN: | 0022-3042 (print) |
Schlagwort: |
|
Sonstiges: |
|