CMOS optical detector system for capillary fluorescence measurements
In: Detectors and associated signal processing (Saint Etienne, 1-2 October 2003)SPIE proceedings series; (2004) S. 154-161
Konferenz
- print, 9 ref
Zugriff:
We present a fluorescence detection system for capillary analysis. It is designed using a CMOS BDJ (Buried Double p-n Junction) detector which can be operated either as a photodiode or as a wavelength-sensitive device. Noise-reduction techniques such as signal pre-amplification and synchronous detection are implemented to boost the sensitivity of measurements. The system indicates fluorescence intensity for concentration determination, and average wavelength of fluorescence spectrum for molecular discrimination. The system has been tested by measuring two widely used fluorophores (FITC and Rhodamine B) in different concentrations. A 407-nm blue laser diode and a 532-nm green YAG compact laser have been respectively employed for their excitation. The illuminated volume inside the capillary is about 5 nl. The best results have been obtained with FITC, enabling as low as 10-10 M to be detectable.
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CMOS optical detector system for capillary fluorescence measurements
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Autor/in / Beteiligte Person: | GALVAN, Jean-Marc ; LU, Guo-Neng ; PITTET, Patrick ; BLUM, Loic ; LECA-BOUVIER, Béatrice |
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Quelle: | Detectors and associated signal processing (Saint Etienne, 1-2 October 2003)SPIE proceedings series; (2004) S. 154-161 |
Veröffentlichung: | Bellingham WA: SPIE, 2004 |
Medientyp: | Konferenz |
Umfang: | print, 9 ref |
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