An adapted ELISA method for differentiating pathogenic from nonpathogenic aPL by a beta 2 glycoprotein I dependency anticardiolipin assay

ABRAMINO LEVY, Roger ; DE MEIS, Ernesto ; et al.

In: State-of-the-art: 11th International Congress on Antiphospholipid Antibodies, 14-18 November 2004, Sydney, Australia, Jg. 114 (2004), Heft 5-6, S. 573-577

Konferenz - print, 27 ref

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With the currently available commercial kits, as well as homemade assays for detecting anticardiolipin antibodies (aCL), it is not possible to discriminate nonpathogenic, beta 2 glycoprotein (GPI)-independent, infection-related antibodies from those of patients with the true autoimmune thrombotic syndrome, known as antiphospholipid syndrome (APS). We devised an assay that is able to differentiate these two types of antibodies by determining the beta 2 GPI requirements to bind in a cardiolipin ELISA. Beta 2 GPI was purified by perchloric acid precipitation, and fixed amounts were used in the dilution solutions of the tested samples that were also tested with no source of beta 2 GPI. The ELISA plates were coated with cardiolipin, as usual, and blocked with a chicken ovalbumin solution. The serum samples had to be highly diluted in order not to have beta 2 GPI from the patient serum. The reaction was detected with alkaline phosphate tablets and developed with pNp in diethanolamine buffer. The adapted ELISA aCL assay described here was able to discriminate infectious [syphilis, hepatitis C virus (HCV), dengue fever, human immunodeficiency virus (HIV) and leprosy] and autoimmune [primary APS and systemic lupus erythematosus (SLE) related APS]. Further testing should be performed to demonstrate that this method consistently differentiates pathogenic antibodies that bind in an aCL ELISA only in the presence of beta 2 GPI.

Titel:	An adapted ELISA method for differentiating pathogenic from nonpathogenic aPL by a beta 2 glycoprotein I dependency anticardiolipin assay
Autor/in / Beteiligte Person:	ABRAMINO LEVY, Roger ; DE MEIS, Ernesto ; PIERANGELI, Silvia
Link:	E-Journal im Bestand der UB Hagen? View record from PASCAL Archive
Zeitschrift:	State-of-the-art: 11th International Congress on Antiphospholipid Antibodies, 14-18 November 2004, Sydney, Australia, Jg. 114 (2004), Heft 5-6, S. 573-577
Quelle:	State-of-the-art: 11 t h International Congress on Antiphospholipid Antibodies, 14-18 November 2004, Sydney, AustraliaThrombosis research 114(5-6):573-577
Veröffentlichung:	New York, NY: Elsevier Science, 2004
Medientyp:	Konferenz
Umfang:	print, 27 ref
ISSN:	0049-3848 (print)
Schlagwort:	Cardiology, blood circulation, phlebology Cardiologie, appareil circulatoire, phlébologie Hematology Hématologie Sciences biologiques et medicales Biological and medical sciences Sciences medicales Medical sciences Cardiologie. Appareil circulatoire Cardiology. Vascular system Vaisseaux sanguins et lymphatiques Blood and lymphatic vessels Maladies vasculaires des membres. Pathologie de la veine cave. Maladies vasculaires diverses Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous Techniques d'exploration et de diagnostic (generalites) Investigative techniques, diagnostic techniques (general aspects) Exploration par ultrasons Ultrasonic investigative techniques Coeur et vaisseaux Cardiovascular system Exploration hémodynamique Investigative techniques of hemodynamics Encéphale Brain Appareil circulatoire pathologie Cardiovascular disease Aparato circulatorio patología Autoimmunité Autoimmunity Autoinmunidad Glycoprotéine Glycoprotein Glicoproteína Anticardiolipin antibodies Antiphosphoilpid antibodies Beta 2 glycoprotein I dependency
Sonstiges:	Nachgewiesen in: PASCAL Archive Sprachen: English Original Material: INIST-CNRS Document Type: Conference Paper File Description: text Language: English Author Affiliations: Department of Rheumatology, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil ; Instituto Nacional do Câncer (INCa-RJ), Rio de Janeiro, Brazil ; Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, GA, United States Rights: Copyright 2004 INIST-CNRS ; CC BY 4.0 ; Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS Notes: Cardiology. Circulatory system ; Scanning and diagnostic techniques (generalities)

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