The distribution and activity of tachykinin-related peptides in the blood-feeding bug, Rhodnius prolixus
In: Invertebrate neuropeptides. VPeptides (New York, NY. 1980) 26(1):43-51; Jg. 26 (2005) 1, S. 43-51
Konferenz
- print, 47 ref
Zugriff:
The invertebrate tachykinin-related peptides (TRPs) with the conserved C-terminal sequence FX1GX2Ramide shows sequence similarity to the vertebrate tachykinins after which they are named, and are hypothesized to be ancestrally related. In this study a polyclonal antiserum generated against a locust tachykinin (LomTK I), was used to demonstrate the presence and describe the distribution of LomTK-like immnoreactivity in the CNS and gut of Rhodnius prolixus. Reverse phase high performance liquid chromatography (RP-HPLC) was used in combination with a sensitive radioimmunoassay (RIA) to demonstrate picomolar amounts of immunoreactive material in the CNS, and femptomolar amounts associated with the hindgut. Furthermore, the results from CNS extracts separated by RP-HPLC, suggest that at least two tachykinin isoforms exist in R. prolixus. A hindgut contraction assay was developed to quantify the myotropic effects of selected LomTKs on R. prolixus hindgut contraction. Both LomTK I and II caused an increase in the frequency of hindgut contractions with EC50 values of 3.6 x 10-8 M and 3.8 x 10-8 M for LomTK I and II. respectively.
Titel: |
The distribution and activity of tachykinin-related peptides in the blood-feeding bug, Rhodnius prolixus
|
---|---|
Autor/in / Beteiligte Person: | KWOK, Rodney ; CHUNG, Doreen ; TE BRUGGE, Victoria ; ORCHARD, Ian |
Link: | |
Quelle: | Invertebrate neuropeptides. VPeptides (New York, NY. 1980) 26(1):43-51; Jg. 26 (2005) 1, S. 43-51 |
Veröffentlichung: | New York, NY: Elsevier Science, 2005 |
Medientyp: | Konferenz |
Umfang: | print, 47 ref |
ISSN: | 0196-9781 (print) |
Schlagwort: |
|
Sonstiges: |
|