[Untitled]
In: San'oeb misaengmul haghoeji, Jg. 26 (1998), Heft 5, S. 400-405
academicJournal
- print, 25 ref
Zugriff:
The ddh gene encoding meso-DAP-dehydrogenase (DDH) involved in the dehydrogenase pathway is essential for high-level lysine production in Brevibacterium lactofermentum. To investigate the effect of the ddh gene amplification on lysine production by B. lactofermentum, we constructed two E. coli - B. lactofermentum shuttle vector, pEB1 and pEB2. The recombinant plasmids, pRK1 and pRK2, carrying the ddh gene were introduced into B. lactofermentum by electroporation. The specific activity of DDH by amplification of the ddh gene was increased 7-fold, and also L-lysine production of B. lactofermentum strains harboring recombinant plasmids were 18∼20% higher than that of the control.
Titel: |
[Untitled]
|
---|---|
Autor/in / Beteiligte Person: | KIM, O.-M ; PARK, S.-H ; LEE, S.-O ; BAE, J.-T ; KIM, H.-J ; LEE, B.-L ; LEE, K.-R |
Link: | |
Zeitschrift: | San'oeb misaengmul haghoeji, Jg. 26 (1998), Heft 5, S. 400-405 |
Veröffentlichung: | Seoul: Korean Society for Applied Microbiology, 1998 |
Medientyp: | academicJournal |
Umfang: | print, 25 ref |
ISSN: | 0257-2389 (print) |
Schlagwort: |
|
Sonstiges: |
|