Prospective isolation and global gene expression analysis of the erythrocyte colony-forming unit (CFU-E)
In: Blood, Jg. 105 (2005), Heft 5, S. 1937-1945
Online
academicJournal
- print, 43 ref
Zugriff:
The erythrocyte colony-forming unit (CFU-E) is a rare bone marrow (BM) progenitor that generates erythrocyte colonies in 48 hours. The existence of CFU-Es Is based on these colonies, but CFU-Es have not been purified prospectively by phenotype. We have separated the non-stem, nonlymphoid compartment (lineage marker [lin]-c-Kit+Sca-1-IL-7Rα-) into interleukin 3 receptor a negative (IL-3Rα-) and IL-3Rα+ subsets. Within IL-3Rα- but not IL-3Rα+ cells we have identified TER119-CD41-CD71+ erythrocyte-committed progenitors (EPs). EPs generate CFU-E colonies at about 70% efficiency and generate reticulocytes in vivo. Depletion of EPs from BM strongly reduces CFU-E frequencies. EPs lack potential for erythrocyte burst-forming unit, megakaryocyte, granulocyte (G), and monocyte (M) colonies, and for spleen colony-forming units. Chronically suppressed erythropoiesis in interferon consensus sequence-binding protein (ICSBP)-deficient BM is associated with reduced frequencies of both the EP population and CFU-E colonies. During phenylhydrazine-induced acute anemia, numbers of both the EP population and CFU-E colonies increase. Collectively, EPs (lin-c-Kit+Sca-1-IL-7Rα-IL-3Rα-CD41-CD71+) account for most, if not all, CFU-E activity in BM. As a first molecular characterization, we have compared global gene expression in EPs and nonerythroid GM progenitors. These analyses define an erythroid progenitor-specific gene expression pattern. The prospective isolation of EPs is an important step to analyze physiologic and pathologic erythropoiesis.
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Prospective isolation and global gene expression analysis of the erythrocyte colony-forming unit (CFU-E)
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Autor/in / Beteiligte Person: | TERSZOWSKI, Grzegorz ; WASKOW, Claudia ; CONRADT, Peter ; LENZE, Dido ; KOENIGSMANN, Jessica ; CARSTANJEN, Dirk ; HORAK, Ivan ; RODEWALD, Hans-Reimer |
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Zeitschrift: | Blood, Jg. 105 (2005), Heft 5, S. 1937-1945 |
Veröffentlichung: | Washington, DC: The Americain Society of Hematology, 2005 |
Medientyp: | academicJournal |
Umfang: | print, 43 ref |
ISSN: | 0006-4971 (print) |
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