Role of serotonin (5-HT) in the antidepressant-like properties of neuropeptide Y (NPY) in the mouse forced swim test
In: International Symposium on Peptide Receptors, Montréal 2004: From gene to therapyPeptides (New York, NY. 1980) 26(8):1394-1400; Jg. 26 (2005) 8, S. 1394-1400
Konferenz
- print, 59 ref
Zugriff:
Neuropeptide Y (NPY) is thought to be implicated in depressive disorders. The mouse forced swim test (FST) is an animal model widely used as a predictor of the efficacy of antidepressant drugs. The present study was undertaken to explore the possible contribution of endogenous serotonin (5-HT) systems in the behavioral effects elicited by NPY in this model. The selective serotonin re-uptake inhibitor (SSRI), fluoxetine, was also tested for comparison. 5-HT was depleted prior to testing by the administration of the tryptophan hydroxylase inhibitor p-chlorophenylalanine (PCPA; 300 mg/kg, i.p., each day for 3 days; control mice received saline-vehicle over the same period). On the fourth day, mice received NPY (3 nmol, I.C.V.), fluoxetine (16 mg/kg, i.p.) or saline injections before testing in the FST. Both NPY and fluoxetine significantly reduced immobility time in saline-treated control animals. Pre-treatment with PCPA significantly blocked the effects of fluoxetine in the FST, confirming the role of endogenous 5-HT. Similarly, pre-treatment with PCPA also significantly attenuated the anti-immobility effects of NPY, thus suggesting a role for 5-HT in the effects of NPY in the FST. Quantitative receptor autoradiography revealed increases in specific [125I][Leu31], Pro14]PYY sites that were sensitive to BIBP3226 (Y1-like sites) in various brain regions. Specific [125I](GR231118 and [125](PYY( 3-36) binding levels were not changed following PCPA treatment, suggesting that depletion of endogenous 5-HT resulted in an apparent increase in the level of Y1 sites in their high-affinity state. Taken together, these results suggest a role for 5-HT-related systems in the antidepressant-like properties of NPY.
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Role of serotonin (5-HT) in the antidepressant-like properties of neuropeptide Y (NPY) in the mouse forced swim test
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Autor/in / Beteiligte Person: | REDROBE, John P ; DUMONT, Yvan ; FOURNIER, Alain ; BAKER, Glen B ; QUIRION, Rémi |
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Quelle: | International Symposium on Peptide Receptors, Montréal 2004: From gene to therapyPeptides (New York, NY. 1980) 26(8):1394-1400; Jg. 26 (2005) 8, S. 1394-1400 |
Veröffentlichung: | New York, NY: Elsevier Science, 2005 |
Medientyp: | Konferenz |
Umfang: | print, 59 ref |
ISSN: | 0196-9781 (print) |
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