A simple and efficient system for green note compound biogenesis by use of certain lipoxygenase and hydroperoxide lyase sources
In: Journal of agricultural and food chemistry (Print), Jg. 53 (2005), Heft 17, S. 6877-6882
academicJournal
- print, 52 ref
Zugriff:
Six-carbon (C6) aldehydes and alcohols are important components of the aroma and flavor of fruits and vegetables. Soybean lipoxygenase (LOX) isozyme LOX 3 was reported not only to produce less 13-hydroperoxides, precursors of C6 aldehydes, but also to convert them to ketodiene products. Here, we examined the effects of LOX 3 on hexenal formation from linolenic acid homogenized with watermelon 13-hydroperoxide lyase (HL)-overexpressing Nicotiana tabacum leaves and soybean acetone powder. Compared to the wild type, which contains LOXs 1, 2, and 3, the elimination of LOX 3 in LOX 1 + 2 facilitates greater production of hexenals. The use of LOX 2 alone yielded the highest hexenal production, while a two-step conversion was required for LOX 1 to produce hexenals at high levels due to different pH optima of the enzymes involved. These results clearly demonstrate that the soybeans lacking LOX 3 in combination with watermelon HL-overexpressing leaf tissues greatly enhance hexenal formation.
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A simple and efficient system for green note compound biogenesis by use of certain lipoxygenase and hydroperoxide lyase sources
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Autor/in / Beteiligte Person: | FUKUSHIGE, Hirotada ; HILDEBRAND, David F |
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Zeitschrift: | Journal of agricultural and food chemistry (Print), Jg. 53 (2005), Heft 17, S. 6877-6882 |
Veröffentlichung: | Washington, DC: American Chemical Society, 2005 |
Medientyp: | academicJournal |
Umfang: | print, 52 ref |
ISSN: | 0021-8561 (print) |
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