Construction of an inducible expression shuttle vector for Laribacter hongkongensis, a novel bacterium associated with gastroenteritis
In: FEMS microbiology letters, Jg. 252 (2005), Heft 1, S. 57-65
Online
academicJournal
- print, 13 ref
Zugriff:
An Escherichia coli-Laribacter hongkongensis shuttle vector (pPW380) was constructed by ligating the 4701-bp EcoRI digested fragment of pHLHK8 to EcoRI digested pBK-CMV. An E. coli-L. hongkongensis inducible expression shuttle vector was further constructed by ligating a 2105-bp fragment that contains the tetracycline repressor and tetracycline-inducible promoter region of pALC2084 to the 8897-bp fragment of pPW380, deletion of the green fluorescent protein gene, and insertion of a multiple cloning site. This inducible expression system was able to express two commonly used reporter genes, the green fluorescent protein gene and the glutathione S-transferase gene, efficiently in E. coli and L. hongkongensis.
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Construction of an inducible expression shuttle vector for Laribacter hongkongensis, a novel bacterium associated with gastroenteritis
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Autor/in / Beteiligte Person: | WOO, Patrick C. Y ; MA, Shirley S. L ; TENG, Jade L. L ; LI, Maria W. S ; KAO, Richard Y. T ; LAU, Susanna K. P ; YUEN, Kwok-Yung |
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Zeitschrift: | FEMS microbiology letters, Jg. 252 (2005), Heft 1, S. 57-65 |
Veröffentlichung: | Oxford: Blackwell, 2005 |
Medientyp: | academicJournal |
Umfang: | print, 13 ref |
ISSN: | 0378-1097 (print) |
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