Characterization of bovine serum albumin-tryptophan interaction by capillary isoelectric focusing with whole column imaging detection
In: 28th International Symposium on Capillary Chromatography and Electrophoresis, Las Vegas, NV, USA, 22-25 May 2005Journal of chromatography 1105(1-2):25-32; Jg. 1105 (2006) 1-2, S. 25-32
Konferenz
- print, 35 ref
Zugriff:
Capillary isoelectric focusing (clEF) with whole column imaging detection (WCID) was explored for the characterization of bovine serum albumin (BSA)-tryptophan interaction, to further understand protein-drug interactions. The BSA-tryptophan interaction was dynamically monitored by cIEF-WCID, to provide the cIEF profiles of the BSA-tryptophan interaction system at different focusing times. Our study demonstrated that the cIEF behavior of BSA can serve as a probe into the study of BSA-tryptophan interaction, through monitoring the change in its clEF profile when the interaction occurred. The study illustrated that the BSA peak split due to the BSA-tryptophan interaction, and the peak of BSA-tryptophan complex was clearly identified in the cIEF electropherograms. By comparing the cIEF profiles of BSA/L-tryptophan and BSA/D-tryptophan, respectively, our study demonstrated that BSA interacted with the enantiomers of tryptophan with a chiral recognition. L-Tryptophan demonstrated a very strong interaction with BSA, while D-tryptophan exhibited a much weaker interaction with BSA. The effects of the BSA concentration, the tryptophan concentration, the focusing time and the incubation time on the BSA-tryptophan interaction were investigated. This study offers a novel approach for the study of protein-drug interactions.
Titel: |
Characterization of bovine serum albumin-tryptophan interaction by capillary isoelectric focusing with whole column imaging detection
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Autor/in / Beteiligte Person: | TAO, BO ; PAWLISZYN, Janusz |
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Quelle: | 28th International Symposium on Capillary Chromatography and Electrophoresis, Las Vegas, NV, USA, 22-25 May 2005Journal of chromatography 1105(1-2):25-32; Jg. 1105 (2006) 1-2, S. 25-32 |
Veröffentlichung: | Amsterdam: Elsevier, 2006 |
Medientyp: | Konferenz |
Umfang: | print, 35 ref |
ISSN: | 0021-9673 (print) |
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