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Recombinant gonadotropins (GTHs), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) of goldfish Carassius auratus were produced by baculovirus in silkworm Bombyx mori larvae. The induction of gonadal maturation is an important aspect of fish aquaculture and the possible biotechnological production of recombinant hormones was examined. cDNAs encoding goldfish GTH subunits (FSHp, LHp and common a) were introduced into the baculovirus, which was infected into silkworm larvae after propagation of the recombinant virus in B. mori culture cells. Hemolymph of silkworm larvae containing recombinant FSH (rFSH) or LH (rLH) was collected, and biological activities of recombinant hormones were analyzed in vivo using goldfish and the bitterling Rhodeus ocellatus ocellatus. Injection of hemolymph containing rFSH or rLH induced milt production in male goldfish. Plasma testosterone levels were increased by rLH in male goldfish and plasma estradiol levels were increased by rFSH in female goldfish. rLH induced ovulation in female bitterling, but rFSH showed weak potency. In conclusion, biologically active goldfish recombinant GTHs were able to be produced in a baculovirus-silkworm larvae system and it is suggested that recombinant fish GTHs produced by this system could be applied for the induction of gonadal development in aquaculture fishes.

Titel:	In vivo biological activity of recombinant goldfish gonadotropins produced by baculovirus in silkworm larvae
Autor/in / Beteiligte Person:	KOBAYASHI, Makito ; MORITA, Tetsuro ; IKEGUCHI, Koki ; YOSHIZAKI, Goro ; SUZUKI, Takeo ; WATABE, Shugo
Link:	E-Journal im Bestand der UB Hagen? View record from PASCAL Archive
Zeitschrift:	Aquaculture (Amsterdam), Jg. 256 (2006), Heft 1-4, S. 433-442
Veröffentlichung:	Amsterdam: Elsevier Science, 2006
Medientyp:	academicJournal
Umfang:	print, 1 p.1/4
ISSN:	0044-8486 (print)
Schlagwort:	Marine biology, limnology Biologie marine, limnologie Ecology Ecologie Environment Environnement Pisciculture, aquaculture, fisheries Pisciculture, aquaculture, pêche Sciences biologiques et medicales Biological and medical sciences Sciences biologiques fondamentales et appliquees. Psychologie Fundamental and applied biological sciences. Psychology Productions animales Animal productions Aquiculture animale Animal aquaculture Généralités General aspects Pisces Vertebrata Virus Activité biologique Biological activity Actividad biológica Aquiculture Aquaculture Acuacultura Baculoviridae Carassius auratus Gonadotrophine Gonadotropin Gonadotropina In vivo Larve Larva Milieu eau douce Freshwater environment Medio agua dulce Goldfish Milt production Ovulation Recombinant hormone Sex steroid
Sonstiges:	Nachgewiesen in: PASCAL Archive Sprachen: English Original Material: INIST-CNRS Document Type: Article File Description: text Language: English Author Affiliations: Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan ; Department of Biology, Division of Natural Sciences, International Christian University, Mitaka, Tokyo 181-8585, Japan ; Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Minato, Tokyo 108-8477, Japan ; Katakura Industries Co. Ltd, Sayama, Saitama 350-1332, Japan Rights: Copyright 2006 INIST-CNRS ; CC BY 4.0 ; Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS Notes: Animal productions

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In vivo biological activity of recombinant goldfish gonadotropins produced by baculovirus in silkworm larvae