Haemonchus contortus : Prokaryotic expression and enzyme activity of recombinant HcSTK, a serine/threonine protein kinase

NIKOLAOU, Sia ; HARTMAN, Dadna ; et al.

In: Experimental parasitology, Jg. 113 (2006), Heft 4, S. 207-214

academicJournal - print, 35 ref

Members of the PAR-1/MARK serine/threonine protein kinase (STK) subfamily are important regulators of the cytoskeleton, and their characterization can provide insights into a number of critical processes relating to the development and survival of an organism. We previously investigated the mRNA expression for and organization of a gene (hcstk) representing HcSTK, an STK from the parasitic nematode Haemonchus contortus. In the present study, a recombinant form of HcSTK was expressed and characterized. Affinity-purified anti-HcSTK antibodies reacted with native HcSTK in protein homogenates extracted from third-stage larvae (L3) of H. contortus and were also used to immunolocalize the protein around the nuclei of ovarian and intestinal tissues of adult H. contortus. The enzyme activity of the recombinant HcSTK protein was also demonstrated. The findings show that recombinant HcSTK is a functional protein kinase, with activity directed to KXGS motifs, consistent with other members of the PAR-I/MARK STK subfamily.

Titel:	Haemonchus contortus : Prokaryotic expression and enzyme activity of recombinant HcSTK, a serine/threonine protein kinase
Autor/in / Beteiligte Person:	NIKOLAOU, Sia ; HARTMAN, Dadna ; NISBET, Alasdair J ; GASSER, Robin B
Link:	E-Journal im Bestand der UB Hagen? View record from PASCAL Archive
Zeitschrift:	Experimental parasitology, Jg. 113 (2006), Heft 4, S. 207-214
Veröffentlichung:	San Diego, CA: Elsevier, 2006
Medientyp:	academicJournal
Umfang:	print, 35 ref
ISSN:	0014-4894 (print)
Schlagwort:	Tropical medicine Médecine tropicale Parasitology Parasitologie Sciences biologiques et medicales Biological and medical sciences Sciences biologiques fondamentales et appliquees. Psychologie Fundamental and applied biological sciences. Psychology Protozoaires Protozoa Cycle évolutif. Relation hôte-agent. Pouvoir pathogène Life cycle. Host-agent relationship. Pathogenesis Enzyme Enzima Helmintha Invertebrata Nemathelminthia Nematoda Transferases Activité enzymatique Enzymatic activity Actividad enzimática Haemonchus contortus Parasite Parásito Protein kinase Sérine Serine Serina Thréonine Threonine Treonina
Sonstiges:	Nachgewiesen in: PASCAL Archive Sprachen: English Original Material: INIST-CNRS Document Type: Article File Description: text Language: English Author Affiliations: Primary Industries Research Victoria (Animal Genetics and Genomics), 475 Mickleham Road, Attwood, Vic. 3049, Australia ; Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Vic. 3030, Australia ; Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian, Scotland EH26 OPZ, United Kingdom ; Biotechnology Research Institute, Macquarie University, Sydney, NSW2109, Australia Rights: Copyright 2006 INIST-CNRS ; CC BY 4.0 ; Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS Notes: Protozoa

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