Induction of cross-reactive immune responses to NTS-DBL-1α/x of PfEMP1 and in vivo protection on challenge with Plasmodium falciparum
In: Vaccine, Jg. 24 (2006), Heft 35-36, S. 6140-6154
academicJournal
- print, 47 ref
Zugriff:
The interactions of Plasmodiumfalciparum infected erythrocytes parasitized red blood cells (pRBC) with endothelial receptors and erythrocytes are mediated by multiple Duffy-binding like (DBL) and cysteine-rich interdomain region (CIDR) domains harboured in the Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP 1). The success of a subunit vaccine based on PfEMP 1 depends on its ability to elicit cross-reactive responses to a substantial number of PfEMP variants. We have here evaluated serological PfEMP cross-reactivity by immunizing rats with phylogenetically diverse recombinant NTS-DBL-1α/x fusion domains from the 3D7 genome parasite emulsified in Montanide ISA 720. Cross-reactivity was elicited to these diverse DBL-1α/x domains as measured by ELISA and by immunoblotting. Employing a novel in vivo model of human infected erythrocyte sequestration, immunized animals were challenged with the FCR3S 1.2 clone and cross-protection in terms of reduction in lung sequestration amounting to approximately 50% was demonstrated. Our results suggest that immunization with phylogenetically distant DBL-1α/x variants, can elicit partial cross-protection to challenge with the parasites harbouring a distant variant. These observations have implications for the design of multi-component vaccines against P. falciparum malaria.
Titel: |
Induction of cross-reactive immune responses to NTS-DBL-1α/x of PfEMP1 and in vivo protection on challenge with Plasmodium falciparum
|
---|---|
Autor/in / Beteiligte Person: | AHUJA, Sanjay ; PETTERSSON, Fredrik ; MULL, Kirsten ; JONSSON, Cathrine ; WAHLGREN, Mats ; QIJUN, CHEN |
Link: | |
Zeitschrift: | Vaccine, Jg. 24 (2006), Heft 35-36, S. 6140-6154 |
Veröffentlichung: | Oxford: Elsevier, 2006 |
Medientyp: | academicJournal |
Umfang: | print, 47 ref |
ISSN: | 0264-410X (print) |
Schlagwort: |
|
Sonstiges: |
|