Dynamics of renal excretion of phenol and major metabolites in the rainbow trout (Oncorhynchus mykiss)
In: Aquatic toxicology, Jg. 45 (1999), Heft 4, S. 265-277
academicJournal
- print, 33 ref
Zugriff:
Renal excretion of phenol (PH), phenyl glucuronide (PG), phenyl sulfate (PS), and hydroquinone (HQ) was determined in 600-900 g rainbow trout (Oncorhynchus mykiss) continuously exposed (144-h) to waterborne PH (5 mg l-1 or 53.2 μmol l-1) in respirometer-metabolism chambers. The 'free' plasma concentration time course was obtained from samples withdrawn from a dorsal aortic cannulae, while the urine concentration time course was acquired using a bladder catheter. Microdialysis and HPLC techniques were used to isolate and identify PH and metabolites. Plasma and urinary PH were at steady-state in 4 h. PG and HQ in both plasma and urine continued to rise throughout 144 h. PS values in plasma reached a maximum around 48 h. while urine PS plateaued near 24 h. Of the net PH gill uptake at steady-state (144 h), as much as 50% was eliminated in the urine (PG = 81. PS = 16, PH = 4%) and the rest extrarenally. PS kinetics suggested the existence of a high affinity/low capacity pathway, while PG production was suggestive of a low affinity/high capacity formation pathway. Rainbow trout glomerular filtration rate (GFR) was 6.10 ml kg-1 h-1, which was 30% higher than the mean urine flow rate (UFR) of 4.28 ml kg-1 h 1. Renal Clearance (Clx) calculated for PH, PG, PS and HQ were 4.12, 8.67. 24.33 and 1.85 ml kg-1 h-1, respectively. Urine Clearance Ratios (Clv/GFR) were 0.69, 1.56, 5.49 and 0.30 for PH. PG, PS and HQ, respectively. The results of this stud y support in vivo tubular secretion in freshwater adapted trout for both PG and PS, and suggest a possible kidney secretory transport maxima for sulfate conjugates.
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Dynamics of renal excretion of phenol and major metabolites in the rainbow trout (Oncorhynchus mykiss)
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Autor/in / Beteiligte Person: | MCKIM, J. M ; KOLANCZYK, R. C ; LIEN, G. J ; HOFFMAN, A. D |
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Zeitschrift: | Aquatic toxicology, Jg. 45 (1999), Heft 4, S. 265-277 |
Veröffentlichung: | Amsterdam: Elsevier Science, 1999 |
Medientyp: | academicJournal |
Umfang: | print, 33 ref |
ISSN: | 0166-445X (print) |
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