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Capillary isoelectric focusing (cIEF) can be a useful tool for the characterization and identification of microbes. Based on the whole column imaging detection (WCID) technique and using plant growth-promoting rhizobacteria (PGPR) as test microbes, we present a two-level cIEF characterization method for the characterization and identification of bacteria. Intact bacteria were first characterized according to their apparent isoelectric points measured by cIEF-WCID and then lysed bacteria were further characterized by cIEF profiling of the intracellular proteins. Cellular clustering was found to be the main experimental barrier for the characterization of intact bacteria. The addition of sodium chloride (100 mM) to the sample mixture was found to be an effective way to reduce clustering. Due to the high efficiency and high resolution ofcIEF-WCID, characterization of bacteria according to their intracellular proteins can be implemented simply and quickly without optimization of the experimental conditions. To improve the detection sensitivity with laser induced fluorescence (LIF)-WCID, the possibility to label bacteria with a non-covalent fluorescent dye, NanoOrange, was explored.

Titel:	Characterization of plant growth-promoting rhizobacteria using capillary isoelectric focusing with whole column imaging detection
Autor/in / Beteiligte Person:	ZHEN, LIU ; SHAN SHAN, WU ; PAWLISZYN, Janusz
Link:	E-Journal im Bestand der UB Hagen? View record from PASCAL Archive
Zeitschrift:	Journal of chromatography, Jg. 1140 (2007), Heft 1-2, S. 213-218
Veröffentlichung:	Amsterdam: Elsevier, 2007
Medientyp:	academicJournal
Umfang:	print, 50 ref
ISSN:	0021-9673 (print)
Schlagwort:	Biochemistry, molecular biology, biophysics Biochimie, biologie moléculaire, biophysique Analytical chemistry Chimie analytique Pharmacology drugs Pharmacologie, galénique Sciences biologiques et medicales Biological and medical sciences Sciences biologiques fondamentales et appliquees. Psychologie Fundamental and applied biological sciences. Psychology Microbiologie Microbiology Bactériologie Bacteriology Morphologie, structure, composition chimique Morphology, structure, chemical composition Agronomie. Sciences du sol et productions vegetales Agronomy. Soil science and plant productions Sciences du sol Soil science Physique, chimie, biochimie et biologie des sols agricoles et forestiers Physics, chemistry, biochemistry and biology of agricultural and forest soils Propriétés chimiques, physicochimiques, biochimiques et biologiques Chemical, physicochemical, biochemical and biological properties Biochimie et biologie Biochemistry and biology Détecteur fluorescence Fluorescence detector Detector fluorescencia Détection Detection Detección Electrophorèse capillaire Capillary electrophoresis Electroforesis capilar Electrophorèse zone Zone electrophoresis Electroforesis zonal Fluorescence induite par laser Laser induced fluorescence Fluorescencia inducida por laser Focalisation isoélectrique Isoelectric focusing Focalización isoeléctrica Formation image Imaging Formación imagen Méthode analyse Analysis method Método análisis Rhizobactérie stimulant croissance plante Plant growth promoting rhizobacteria Rizobacteria estimulante crecimiento planta Bacteria Capillary isoelectric focusing Characterization Laser-induced fluorescence Whole column imaging detection
Sonstiges:	Nachgewiesen in: PASCAL Archive Sprachen: English Original Material: INIST-CNRS Document Type: Article File Description: text Language: English Author Affiliations: Department of Chemistry, Nanjing University, Nanjing 210093, China ; Department of Biology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada ; Department of Chemistry, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada Rights: Copyright 2007 INIST-CNRS ; CC BY 4.0 ; Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS Notes: Agronomy. Soil sciences and vegetal productions ; Microbiology

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Characterization of plant growth-promoting rhizobacteria using capillary isoelectric focusing with whole column imaging detection