Strain-typing of Lentinula edodes in China with inter simple sequence repeat markers
In: Applied microbiology and biotechnology, Jg. 74 (2007), Heft 1, S. 140-145
Online
academicJournal
- print, 1/2 p
Zugriff:
To validate strain typing by inter simple sequence repeat (ISSR) analysis in Lentinula edodes cultivars, 17 Chinese L. edodes strains including 15 cultivated strains cultivated on a large scale and two wild strains were analyzed with the ISSR technique. With the use of two ISSR primers, a total of 32 DNA products were detected, of which, 31 DNA products (96.9% of the detected products) were polymorphic between two or more strains. The profiles of those two primers could be employed to differentiate all of the tested strains. A cluster analysis based on ISSR data revealed that the 17 strains could be classified into two distinct groups. One group consisted of eight strains in which the cultivated strains were H (high-temperature)-type or B (broad-temperature)-type, and the other group comprised cultivated strains that were of the L (low-temperature)-type or M (medium-temperature)-type. In contrast to the two wild strains, the genetic diversity of 15 cultivated strains was very rich based on a similarity coefficient analysis.
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Strain-typing of Lentinula edodes in China with inter simple sequence repeat markers
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Autor/in / Beteiligte Person: | RUIYING, ZHANG ; CHENYANG, HUANG ; SUYUE, ZHENG ; JINXIA, ZHANG ; TZI BUN, NG ; RUIBO, JIANG ; XUEMEI, ZUO ; HEXIANG, WANG |
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Zeitschrift: | Applied microbiology and biotechnology, Jg. 74 (2007), Heft 1, S. 140-145 |
Veröffentlichung: | Berlin: Springer, 2007 |
Medientyp: | academicJournal |
Umfang: | print, 1/2 p |
ISSN: | 0175-7598 (print) |
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