Inhibition of Epstein Barr Virus LMP1 gene expression in B lymphocytes by antisense oligonucleotides : Uptake and efficacy of lipid-based and receptor-mediated delivery systems

GALLETTI, Roberta ; MASCIARELLI, Silvia ; et al.

In: Antiviral research, Jg. 74 (2007), Heft 2, S. 102-110

academicJournal - print, 3/4 p

Epstein Barr Virus (EBV), is associated with an increasing number of lymphoid and epithelial malignancies. Among the genes expressed by EBV during latency, LMP1 plays a key role for growth transformation and immortalization of B lymphocytes. We have previously shown that antisense oligonucleotides (ONs) directed to LMP1 mRNA, effectively suppressed LMP1 gene expression and substantially reduced proliferation of the infected cells. The use of antisense phosphodiester oligonucleotides as therapeutic agents is limited by inefficient cellular uptake and intracellular transport to the target mRNA. We tested the ability of three cationic carriers internalized by different pathways, to increase the delivery of anti-LMP1-ON to their site of action in EBV-infected B lymphocytes. We report here that liposomes, dendrimers or transferrin-polylysine-conjugated ON were internalized by the cells at an extent several fold higher than that of the naked oligomers. However, only the delivery system exploiting the transferrin receptor pathway of internalization, was able to vectorize biologically active antisense LMP1-ON.

Titel:	Inhibition of Epstein Barr Virus LMP1 gene expression in B lymphocytes by antisense oligonucleotides : Uptake and efficacy of lipid-based and receptor-mediated delivery systems
Autor/in / Beteiligte Person:	GALLETTI, Roberta ; MASCIARELLI, Silvia ; CONTI, Cinzia ; MATUSALI, Giulia ; DI RENZO, Livia ; MESCHINI, Stefania ; ARANCIA, Giuseppe ; MANCINI, Carlo ; MATTIA, Elena
Link:	E-Journal im Bestand der UB Hagen? View record from PASCAL Archive
Zeitschrift:	Antiviral research, Jg. 74 (2007), Heft 2, S. 102-110
Veröffentlichung:	Amsterdam: Elsevier, 2007
Medientyp:	academicJournal
Umfang:	print, 3/4 p
ISSN:	0166-3542 (print)
Schlagwort:	Microbiology, infectious diseases Microbiologie, maladies infectieuses Pharmacology drugs Pharmacologie, galénique Virology Virologie Sciences biologiques et medicales Biological and medical sciences Sciences medicales Medical sciences Pharmacologie. Traitements medicamenteux Pharmacology. Drug treatments Antibiotiques. Antiinfectieux. Antiparasitaires Antibiotics. Antiinfectious agents. Antiparasitic agents Antiviraux Antiviral agents Hemopathies Hematologic and hematopoietic diseases Leucémies. Lymphomes malins. Réticuloses malignes. Hématodermies. Splénomégalie myéloïde Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Gammaherpesvirinae Herpesviridae Hémopathie maligne Malignant hemopathy Hemopatía maligna Lymphoprolifératif syndrome Lymphoproliferative syndrome Linfoproliferativo síndrome Polymère ramifié Branched polymer Polímero ramificado Virus Antiviral DNA Expression génique Gene expression Expresión genética Internalisation Internalization Internalización Liposome Liposoma Lymphocyte B B-Lymphocyte Linfocito B Lymphome Lymphoma Linfoma Oligonucléotide antisens Antisense oligonucleotide Oligonucleótido antisentido Site action Site of action Lugar acción Structure dendritique Dendritic structure Estructura dendrítica Système administration Delivery system Sistema administración Vecteur Vector Virus Epstein Barr Epstein Barr virus Dendrimère Dendrimer Dendrímero Gène LMP 1 Récepteur TfR Transferrin receptor Antisense oligonucleotides DNA vectors EBV LMP1
Sonstiges:	Nachgewiesen in: PASCAL Archive Sprachen: English Original Material: INIST-CNRS Document Type: Article File Description: text Language: English Author Affiliations: Department of Public Health Sciences, University La Sapienza , P. le A. Moro 5, 00185 Rome, Italy ; Department of Experimental Medicine and Pathology, V. le Regina Elena 324, 00161 Rome, Italy ; Department of Technology and Health, Istituto Superiore di Sanità, V. le Regina Elena 299, 00161 Rome, Italy Rights: Copyright 2007 INIST-CNRS ; CC BY 4.0 ; Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS Notes: Blood diseases ; Pharmacological treatments

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