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The use of the conventional 5-day biochemical oxygen demand (BOD5) method in BOD determination is greatly hampered by its time-consuming sampling procedure and its technical difficulty in the handling of a large pool of wastewater samples. Thus, it is highly desirable to develop a fast and high-throughput biosensorfor BOD measurements. This paper describes the construction of a microplate-based biosensor consisting of an organically modified silica (ORMOSIL) oxygen sensing film for high-throughput determination of BOD in wastewater. The ORMOSIL oxygen sensing film was prepared by reacting tetramethoxysilane with dimethyldimethoxysilane in the presence of the oxygen-sensitive dye tris(4,7-diphenyl-1,10-phenanthroline)ruthenium-(II) chloride. The silica composite formed a homogeneous, crack-free oxygen sensing film on polystyrene microtiter plates with high stability, and the embedded ruthenium dye interacted with the dissolved oxygen in wastewater according to the Stern-Volmer relation. The bacterium Stenotrophomonas maltophilia was loaded into the ORMOSIL/ PVA composite (deposited on the top of the oxygen sensing film) and used to metabolize the organic compounds in wastewater. This BOD biosensor was found to be able to determine the BOD values of wastewater samples within 20 min by monitoring the dissolved oxygen concentrations. Moreover, the BOD values determined by the BOD biosensor were in good agreement with those obtained by the conventional BOD5 method.

Titel:	High-throughput determination of biochemical oxygen demand (BOD) by a microplate-based biosensor
Autor/in / Beteiligte Person:	PANG, Hei-Leung ; KWOK, Nga-Yan ; CHAN, Pak-Ho ; YEUNG, Chi-Hung ; WAIHUNG, LO ; WONG, Kwok-Yin
Link:	E-Journal im Bestand der UB Hagen? View record from PASCAL Archive
Zeitschrift:	Environmental science & technology, Jg. 41 (2007), Heft 11, S. 4038-4044
Veröffentlichung:	Washington, DC: American Chemical Society, 2007
Medientyp:	academicJournal
Umfang:	print, 32 ref
ISSN:	0013-936X (print)
Schlagwort:	Biotechnology Biotechnologies Ecology Ecologie Energy Énergie Environment Environnement Pollution Sciences exactes et technologie Exact sciences and technology Sciences appliquees Applied sciences Traitement et pollution des eaux Water treatment and pollution Eaux usées Wastewaters Sciences biologiques et medicales Biological and medical sciences Sciences biologiques fondamentales et appliquees. Psychologie Fundamental and applied biological sciences. Psychology Biotechnologie Méthodes. Procédés. Technologies Methods. Procedures. Technologies Méthodes et appareillages divers Various methods and equipments Biodétecteurs. Biocapteurs Biosensors Bactérie Bacteria Pseudomonadaceae Pseudomonadales Appareil mesure Measuring instrument Aparato medición Biodétecteur Biosensor Biodetector DBO Biological oxygen demand Eau usée Waste water Agua residual Inoculation Inoculación Matériau composite Composite material Material compuesto Matériau modifié Modified material Material modificado Micropolluant Micropollutant Microcontaminante Méthode contrôle Control method Método control Performance Rendimiento Ruthénium complexe Ruthenium complex Rutenio complejo Silane organique Organic silane Silano orgánico Siloxane organique Organic siloxane Siloxano orgánico Stenotrophomonas maltophilia Styrène polymère Styrene polymer Estireno polímero Surveillance Vigilancia
Sonstiges:	Nachgewiesen in: PASCAL Archive Sprachen: English Original Material: INIST-CNRS Document Type: Article File Description: text Language: English Author Affiliations: Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hunghom, Kowloon, Hong-Kong Rights: Copyright 2007 INIST-CNRS ; CC BY 4.0 ; Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS Notes: Biotechnology ; Pollution

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High-throughput determination of biochemical oxygen demand (BOD) by a microplate-based biosensor