Low-molecular-weight protein (LMP)2/LMP7 abnormality underlies the downregulation of human leukocyte antigen class i antigen in a hepatocellular carcinoma cell line
In: Journal of gastroenterology and hepatology, Jg. 22 (2007), Heft 7, S. 1155-1161
Online
academicJournal
- print, 29 ref
Zugriff:
Background: Tumor cells may alter the expression of numerous components involved in antigen-processing machinery to decrease human leukocyte antigen (HLA) class I expression, allowing the tumor cells to escape immune surveillance. The purpose of the present study was to investigate the involvement of these components in the downregulation of HLA class I expression in human hepatocellular carcinoma cell line BEL7404. Methods: Expression of HLA-I and antigen presentation-related genes were analyzed by flow cytometry and polymerase chain reaction. The HLA class I-deficient BEL7404 cell was transfected with the low-molecular-weight protein (LMP) 2 and LMP7 gene and were analyzed by flow cytometry for restoration of surface HLA class I expression. Results: The BEL7404 cells downregulated the expression of HLA class I antigen and lacked expression of LMP2 and LMP7. Interferon (IFN)-y treatment increased the expression of LMP2 but not LMP7. The LMP2-transfected BEL7404 cells or LMP2 and LMP7-cotransfected cells restored surface HLA class I expression while LMP7-transfected cells did not. However, in IFN-y-treated BEL7404 cells, transfection with the LMP7 gene induced more HLA class I expression than mock transfection. Conclusions: The LMP2 gene was required for the expression of HLA class I molecules in BEL7404. The LMP7 was not the major reason for loss of HLA class I in BEL7404 cells, although the supply of exogenous LMP7 could increase surface expression of HLA class I antigen.
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Low-molecular-weight protein (LMP)2/LMP7 abnormality underlies the downregulation of human leukocyte antigen class i antigen in a hepatocellular carcinoma cell line
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Autor/in / Beteiligte Person: | SHEN, Yu-Qing ; ZHANG, Jian-Qiong ; MEI, XIA ; MIAO, Feng-Qing ; SHAN, Xiang-Nian ; WEI, XIE |
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Zeitschrift: | Journal of gastroenterology and hepatology, Jg. 22 (2007), Heft 7, S. 1155-1161 |
Veröffentlichung: | Carlton: Blackwell Science, 2007 |
Medientyp: | academicJournal |
Umfang: | print, 29 ref |
ISSN: | 0815-9319 (print) |
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