Towards Agrobacterium-mediated transformation of Hippeastrum x chmielii chm : Identification of appropriate selection media
In: Proceedings of the Vth International Symposium on In Vitro Culture and Horticultural Breeding (Debrecen, Hungary, September 12-17, 2004)Acta horticulturae; (2006) S. 795-800
Konferenz
- print, 1 p.1/4 2
Zugriff:
[...] Successful plant transformation requires efficient regeneration and selection system and these were developed for a new hybrid Hippeastrum x chmielii Chm. This hybrid shows very vigorous growth and flowers without undergoing a quiescent phase. In preparation of transformation experiments the aim of this study was to establish appropriate media for selecting transgenic cells/regenerants. Therefore, the effect of different concentrations of glufosinate (syn. phosphinothricin) on plant regeneration and propagation were analyzed. Glufosinate will be used in transformation experiments to select the cells expressing the pat gene. In addition the influence of cefotaxim, an antibiotic used for suppression of agrobacteria after co-culture, on the selective efficiency of glufosinate was tested.[...].
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Towards Agrobacterium-mediated transformation of Hippeastrum x chmielii chm : Identification of appropriate selection media
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Autor/in / Beteiligte Person: | ILEZUK, A ; ŁUKASZEWSKA, A ; WINKELMANN, T ; SEREK, M |
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Quelle: | Proceedings of the Vth International Symposium on In Vitro Culture and Horticultural Breeding (Debrecen, Hungary, September 12-17, 2004)Acta horticulturae; (2006) S. 795-800 |
Veröffentlichung: | Leuven: International Society for Horticultural Sciences, 2006 |
Medientyp: | Konferenz |
Umfang: | print, 1 p.1/4 2 |
ISSN: | 0567-7572 (print) |
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