Detection of human herpesviruses HHV-6, HHV-7 and HHV-8 in whole blood by real-time PCR using the new CMV, HHV-6, 7, 8 R-gene™ kit
In: Journal of virological methods, Jg. 149 (2008), Heft 2, S. 285-291
academicJournal
- print, 3/4 p
Zugriff:
Human herpesviruses 6 (HHV-6), 7 (HHV-7) and 8 (HHV-8) are lymphotropic herpesviruses that may cause opportunistic diseases in immunosuppressed patients such as transplant or AIDS patients. The new commercial CMV HHV-6, 7, 8 R-gene™ kit (Argene, Varilhes, France) for the simultaneous quantitation of HHV-6 and qualitative detection of HHV-7 and HHV-8 was evaluated using whole blood samples (respectively, n = 175, 100 and 161) and using different extraction and real-time PCR platforms in two Centers A and B. In comparison with HHV-6 in-house real-time PCR the commercial kit showed agreements of 96% (n=75) and 85% (n = 100) in A and B, respectively, with significant Spearman's correlation between both techniques (in A: r=Q.91 [p< 0.001]; in B: r=0.70 [p< 0.001]). The Bland-Altman test results and prospective monitoring of patients confirmed the accuracy of these HHV-6 real-time PCR techniques. The agreement between the in-house HHV-7 PCR and commercial kit was of 86% (n = 100). In comparison with in-house HHV-8 real-time PCRs, the commercial kit showed agreements of 100% (n = 61) and 93.7% (n = 96) in A and B, respectively. These results demonstrate that the new commercial CMV HHV-6, 7, 8 R-gene™ kit was an efficient and reliable tool for the diagnosis of herpesvirus 6, 7, 8 infections.
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Detection of human herpesviruses HHV-6, HHV-7 and HHV-8 in whole blood by real-time PCR using the new CMV, HHV-6, 7, 8 R-gene™ kit
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Autor/in / Beteiligte Person: | DEBACK, C ; AGBALIKA, F ; SCIEUX, C ; MARCELIN, A. G ; GAUTHERET-DEJEAN, A ; CHEROT, J ; HERMET, L ; ROGER, O ; AGUT, H |
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Zeitschrift: | Journal of virological methods, Jg. 149 (2008), Heft 2, S. 285-291 |
Veröffentlichung: | Amsterdam; London; New York, NY: Elsevier, 2008 |
Medientyp: | academicJournal |
Umfang: | print, 3/4 p |
ISSN: | 0166-0934 (print) |
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