Pressurized CEC of neutral and charged solutes using silica monolithic stationary phases functionalized with 3-(2-aminoethylamino)propyl ligands

GUIHUA, HUANG ; WENCAN, ZENG ; et al.

In: Journal of separation science, Jg. 31 (2008), Heft 12, S. 2244-2251

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A novel silica monolithic stationary phase functionalized with 3-(2-aminoethylamino)propyl ligands for pressurized CEC has been presented. The monolithic capillary columns were prepared by a sol-gel process in 75 μm id fused-silica capillaries and followed by a chemical modification. The diamino groups on the surface of the stationary phase are meant to generate the chromatographic surface and a substantial anodic EOF as well as to provide electrostatic interaction sites for charged solutes. The electrochromatographic characterization and column performance were evaluated by a variety of neutral and charged solutes. It was observed that the anodic EOF for the diamine-bonded monolith was greatly affected by the reaction time with 3-(2-aminoethylamino)propyltrimethoxysilane and the PEG amount in the sol-gel reaction mixture in addition to the mobile phase conditions. The monolithic stationary phase exhibited hydrophilic interaction chromatographic behavior toward neutral solutes. Good separations of various solutes including phenols, nucleic acid bases, nucleosides and nucleotides were achieved under different experimental conditions. Fast and efficient separations were obtained with high plate counts reaching more than 130000 plates/m.

Titel:	Pressurized CEC of neutral and charged solutes using silica monolithic stationary phases functionalized with 3-(2-aminoethylamino)propyl ligands
Autor/in / Beteiligte Person:	GUIHUA, HUANG ; WENCAN, ZENG ; QIUYAN, LIAN ; ZENGHONG, XIE
Link:	Full Text Finder (Volltext) E-Journal im Bestand der UB Hagen? View record from PASCAL Archive
Zeitschrift:	Journal of separation science, Jg. 31 (2008), Heft 12, S. 2244-2251
Veröffentlichung:	Weinheim: Wiley, 2008
Medientyp:	academicJournal
Umfang:	print, 43 ref
ISSN:	1615-9306 (print)
Schlagwort:	Biochemistry, molecular biology, biophysics Biochimie, biologie moléculaire, biophysique Analytical chemistry Chimie analytique Pharmacology drugs Pharmacologie, galénique Sciences exactes et technologie Exact sciences and technology Chimie Chemistry Méthodes chromatographiques et méthodes physiques associées à la chromatographie Chromatographic methods and physical methods associated with chromatography Autres méthodes chromatographiques Other chromatographic methods Sciences biologiques et medicales Biological and medical sciences Sciences biologiques fondamentales et appliquees. Psychologie Fundamental and applied biological sciences. Psychology Biochimie analytique, structurale et metabolique Analytical, structural and metabolic biochemistry Acides nucléiques Nucleic acids Généralités, méthodes d'étude General aspects, investigation methods Caractérisation Characterization Caracterización Colonne monolithique Monolithic column Columna monolítica Condition opératoire Operating conditions Condición operatoria Dérivé de la purine Purine derivatives Purina derivado Dérivé de la pyrimidine Pyrimidine derivatives Pirimidina derivado Effet pression Pressure effect Efecto presión Efficacité plateau Plate efficiency Eficacia plato Electrochromatographie Electrochromatography Electrocromatografía Evaluation performance Performance evaluation Evaluación prestación Microscopie électronique balayage Scanning electron microscopy Microscopía electrónica barrido Mobilité électrophorétique Electrophoretic mobility Movilidad electroforética Morphologie Morphology Morfología Méthode analyse Analysis method Método análisis Nucléoside Nucleoside Nucleósido Nucléotide Nucleotide Nucleótido Phase stationnaire greffée Bonded stationary phase Fase estationaria injertada Phénols Phenols Fenoles Procédé sol gel Sol gel process Procedimiento sol gel Préparation Preparation Preparación Résolution pic Peak resolution Resolución cresta Structure surface Surface structure Estructura superficie Greffage aminoéthylaminopropyle Diamine-bonded EOF Pressurized CEC Silica monolithic column Stationary phases
Sonstiges:	Nachgewiesen in: PASCAL Archive Sprachen: English Original Material: INIST-CNRS Document Type: Article File Description: text Language: English Author Affiliations: Department of Chemistry, Fuzhou University, Fuzhou, China Rights: Copyright 2008 INIST-CNRS ; CC BY 4.0 ; Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS Notes: Analytical chemistry ; Analytical, structural and metabolic biochemistry

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