Myo -inositol facilitators loin and lolT2 enhance D-mannitol formation from D-fructose in Corynebacterium glutamicum
In: FEMS microbiology letters, Jg. 290 (2009), Heft 2, S. 227-235
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Zugriff:
Reduction of D-fructose to D-mannitol by whole-cell biotransformation with recombinant resting cells of Corynebacterium glutamicum ATCC13032 requires the coexpression of mdh and fdh, which encode mannitol and formate dehydrogenases, respectively. However, D-mannitol formation is limited by the uptake of D-fructose in its unphosphorylated form, because additional expression of the sugar facilitator from Zymomonas mobilis resulted in a significantly increased productivity. Here we identified similarities of the myo-inositol transporters lolT1 and IolT2 of C. glutamicum to the sugar facilitator of Z. mobilis. The myo-inositol transporter genes were both individually overexpressed and deleted in recombinants expressing mdh and fdh. Biotransformation experiments showed that the presence and absence, respectively, of IolT1 and IolT2 significantly influenced D-mannitol formation, indicating a D-fructose transport capability of these transporters. For further evidence, a C. glutamicum AptsF mutant unable to grow with D-fructose was complemented with a heterologous fructokinase gene. This resulted in restoration of growth with D-fructose. Using overexpressed iolT1, mdh and fdh, D-mannitol formation obtained with C. glutamicum was 34.2 g L-1, as opposed to 16gL-1 formed by the strain overexpressing only mdh and fdh, showing the suitability of myo-inositol transporters for D-fructose uptake to obtain D-mannitol formation by whole-cell biotransformation with C. glutamicum.
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Myo -inositol facilitators loin and lolT2 enhance D-mannitol formation from D-fructose in Corynebacterium glutamicum
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Autor/in / Beteiligte Person: | BÄUMCHEN, Carsten ; KRINGS, Eva ; BRINGER, Stephanie ; EGGELING, Lothar ; SAHM, Hermann |
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Zeitschrift: | FEMS microbiology letters, Jg. 290 (2009), Heft 2, S. 227-235 |
Veröffentlichung: | Oxford: Wiley-Blackwell, 2009 |
Medientyp: | academicJournal |
Umfang: | print, 1 p.1/2 |
ISSN: | 0378-1097 (print) |
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