Maturing dynamics of surface microflora in Fontina PDO cheese studied by culture-dependent and -independent methods
In: Journal of applied microbiology (Print), Jg. 106 (2009), Heft 1, S. 278-287
Online
academicJournal
- print, 2 p
Zugriff:
Aims: To study the evolution of rind microbial communities in Fontina PDO cheese. Methods and Results: Four batches were examined for their surface microflora during ripening, carried out in two different maturing caves, at Ollomont and Pré-Saint-Didier, Aosta Valley region, Northwest of Italy. Culture-dependent methodologies were combined with culture-independent analysis (PCR-DGGE). Yeasts were found to increase from 103 to 106 CFU cm-2 in 28 days, with consequent rise of surface pH, which allowed the growth of salt-tolerant bacteria, in particular coryneforms which reached 109 CFU cm-2 at the end of 3 months. Coagulase-negative cocci and lactic acid bacteria reached 107 CFU cm-2 in the same period. Debaryomyces hansenii and Candida sake were the species more constantly present throughout the whole maturing process. As early as after 1 day since manufacture, Lactococcus lactis subsp. lactis and Streptococcus thermophilus were detected on cheese rinds. Arthrobacter nicotianae, Brevibacterium casei and Corynebacterium glutamicum were found after 7-28 days. Conclusions: According to cluster analysis of DGGE profiles, the maturing environment seemed to influence the dynamics of microbial groups on Fontina surfaces. Significance and Impact of the Study: These results represent a first picture of micro-organisms colonizing Fontina PDO rinds. Further studies are in progress to better understand the origin of this surface microflora and to formulate surface starters.
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Maturing dynamics of surface microflora in Fontina PDO cheese studied by culture-dependent and -independent methods
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Autor/in / Beteiligte Person: | DOLCI, P ; BARMAZ, A ; ZENATO, S ; PRAMOTTON, R ; ALESSANDRIA, V ; COCOLIN, L ; RANTSIOU, K ; AMBROSOLI, R |
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Zeitschrift: | Journal of applied microbiology (Print), Jg. 106 (2009), Heft 1, S. 278-287 |
Veröffentlichung: | Oxford: Blackwell, 2009 |
Medientyp: | academicJournal |
Umfang: | print, 2 p |
ISSN: | 1364-5072 (print) |
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