Trypanosoma cruzi: Altered parasites after in vitro treatment with gangliosides, a therapeutic agent in experimental Chagas' disease
In: Experimental parasitology, Jg. 122 (2009), Heft 3, S. 218-225
academicJournal
- print, 3/4 p
Zugriff:
Biochemical and structural modifications were investigated in axenic cultured Trypanosoma cruzi after treatment with gangliosides. Fluorescence anisotropy showed dose dependent increments in parasite membranes of ganglioside treated epimastigotes. NADP-GDH activity increased in parasites treated at day 4 (13%), 7 (137.2%), and 14 (28.50%) while NAD-MDH but decreased from day 7 to 21 (―5.74%, ―32.22%, ―27.92%). Treated parasites presented electron-lucent vacuoles opposite to the cytostoma, multilamellar bodies and dilated mitochondrion cristae, disorganized kinetoplast and altered heterochromatin structure. Gangliosides inhibited fusogenic ability (80%) and PLA2 activity (>75%) from the parasite. The same occurred with anti-PLA2 antibodies. Trypomastigotes suffered loss of cytoplasmic material and organelles when GM1 was present in culture medium. We propose that exogenous gangliosides produced: altered lipid order, inhibited membrane enzymes, the parasite energy source shifted from glucose to amino acids, ending on a structural transformation which signals parasite cell death.
Titel: |
Trypanosoma cruzi: Altered parasites after in vitro treatment with gangliosides, a therapeutic agent in experimental Chagas' disease
|
---|---|
Autor/in / Beteiligte Person: | COSSY ISASI, S ; RODRIGUEZ, M ; PEREIRA, B. M. I ; DIAZ-IUJAN, C ; FRETES, R. E ; HAÜEN, D. I |
Link: | |
Zeitschrift: | Experimental parasitology, Jg. 122 (2009), Heft 3, S. 218-225 |
Veröffentlichung: | Amsterdam: Elsevier, 2009 |
Medientyp: | academicJournal |
Umfang: | print, 3/4 p |
ISSN: | 0014-4894 (print) |
Schlagwort: |
|
Sonstiges: |
|