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α-Thalassaemia 1 genetic disorder occurs when there is a deletion of two linked α-globin genes. The interaction between these abnormal genes leads to the most severe type of thalassaemia disease, haemoglobin (Hb) Bart's hydrops fetalis. The identification of α-thalassaemia 1 carriers and genetic counselling are essential for the prevention and control of severe thalassaemia diseases. In this study, we have developed a rapid screening method for identifying α-thalassaemia 1. A sandwich-type immunochromatographic (IC) strip test was developed, using the generated monoclonal anti-Hb Bart's antibody, to trace the Hb Bart's in haemolysates. When assayed by our IC strip test, all α-thalassaemia 1, HbH disease, HbH-Constant Spring (H-CS) disease, HbH-CS and heterozygous HbE (CSEA) Bart's disease, and homozygous α-thalassaemia 2 showed positive results. No false negative results were observed in these blood samples. In α-thalassaemia 2 heterozygotes, 83% of them showed positive reactivity. Among HbE (both homozygotes and heterozygotes), β-thalassaemia (heterozygotes, homozygotes and β-thalassaemia/HbE) and normal subjects, the IC strip test revealed negative reactivity of 100, 85 and 97%, respectively. These results indicate that this novel immunodiagnostic kit, in combination with red blood cell indices, is suitable for screening and ruling out mass populations for the presence of α-thalassaemia 1.

Titel:	Simple method for screening of α-thalassaemia 1 carriers
Autor/in / Beteiligte Person:	TAYAPIWATANA, Chatchai ; KUNTARUK, Surakit ; TATU, Thanusak ; CHIAMPANICHAYAKUL, Sawitree ; MUNKONGDEE, Thongperm ; WINICHAGOON, Pranee ; FUCHAREON, Suthat ; KASINRERK, Watchara
Link:	Full Text Finder (Volltext) E-Journal im Bestand der UB Hagen? View record from PASCAL Archive
Zeitschrift:	International journal of hematology, Jg. 89 (2009), Heft 5, S. 559-567
Veröffentlichung:	Heidelberg: Springer, 2009
Medientyp:	academicJournal
Umfang:	print, 20 ref
ISSN:	0925-5710 (print)
Schlagwort:	Hematology Hématologie Sciences biologiques et medicales Biological and medical sciences Sciences medicales Medical sciences Hemopathies Hematologic and hematopoietic diseases Maladies des hématies Diseases of red blood cells Anémies. Hémoglobinopathies Anemias. Hemoglobinopathies Anémie hémolytique Hemolytic anemia Anemia hemolítica Hémoglobinopathie Hemoglobinopathy Hemoglobinopatía Hémopathie Hemopathy Hemopatía Maladie héréditaire Genetic disease Enfermedad hereditaria Anticorps monoclonal Monoclonal antibody Anticuerpo monoclonal Dépistage Medical screening Descubrimiento Hybridome Hybridoma Hibridoma Hematología Méthode Method Método Thalassémie α α-Thalassemia Talasemia α Immunochromatographic strip test Thalassaemia α-Thalassaemia 1
Sonstiges:	Nachgewiesen in: PASCAL Archive Sprachen: English Original Material: INIST-CNRS Document Type: Article File Description: text Language: English Author Affiliations: Biomedical Technology Research Center, National Center for Genetic Engineering and Biotechnology, National Sciences and Technology Development Agency at the Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand ; Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand ; Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand ; Thalassemia Research Center, Institute of Sciences and Technology for Research and Development, Mahidol University, Salaya, Nakornpathom 73170, Thailand Rights: Copyright 2009 INIST-CNRS ; CC BY 4.0 ; Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS Notes: Blood diseases

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Simple method for screening of α-thalassaemia 1 carriers