Synthesis of Maleimide-End-Functionalized Star Polymers and Multimeric Protein-Polymer Conjugates
In: Macromolecules (Print), Jg. 42 (2009), Heft 21, S. 8028-8033
academicJournal
- print, 61 ref
Zugriff:
Protein—polymer conjugates exhibit superior properties to unmodified Proteins, generating a high demand for these materials in the fields ofmedicine, biotechnology, and nanotechnology. Multimeric conjugates are predicted to surpass the activity ofmonomeric conjugates. Herein, we report a straightforward method to synthesize multimeric polymer conjugates. Four-armed poly(N-isopropylacrylamide) (pNIPAAm) was synthesized by reversible addition—fragmentation chain transfer (RAFT) polymerization in the presence of a tetrafunctionalized trithiocarbonate chain transfer agent (CTA). The polymer molecular weight, architecture, and polydispersity index (PDI) were verified by gel permeation chromatography (GPC). dynamic light scattering gel permeation chromatography (DLS-GPC), and matrix-assisted laser resorption/ ionization time-of-flight (MALDI-TOF) mass spectrometry. This approach afforded well-defined polymers (PDI's < 1.06) and the ability to target various molecular weights. Maleimide functional groups were introduced at the chain ends by heating the polymers in the presence of a furan-protected azo-initiator. This allowed for site-specific conjugation of V131CT4 lysozyme to the polymers to generate multimeric protein-polymer conjugates. MALDI-TOF mass spectrometry, electrospray ionization gas-phase electrophoretic mobility macromolecule analysis (ESI-GEMMA), gel electrophoresis, and liquid chromatography tandem mass spectrometry (LC-MS/MS) of the trypsin digests demonstrated that multimeric protein—polymer conjugates had formed. This simple strategy provides ready access to star protein-polymer conjugates for application in the fields of drug discovery, drug delivery, and nanotechnology.
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Synthesis of Maleimide-End-Functionalized Star Polymers and Multimeric Protein-Polymer Conjugates
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Autor/in / Beteiligte Person: | LEI, TAO ; KADDIS, Catherine S ; OGORZALEK LOO, Rachel R ; GROVER, Gregory N ; LOO, Joseph A ; MAYNARD, Heather D |
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Zeitschrift: | Macromolecules (Print), Jg. 42 (2009), Heft 21, S. 8028-8033 |
Veröffentlichung: | Washington, DC: American Chemical Society, 2009 |
Medientyp: | academicJournal |
Umfang: | print, 61 ref |
ISSN: | 0024-9297 (print) |
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